• Publications
  • Influence
Human mitochondrial DNA polymerase holoenzyme: reconstitution and characterization.
We have reconstituted the holoenzyme of the human mitochondrial DNA polymerase from cloned and overexpressed catalytic and accessory subunits. We have examined the polymerization activity of theExpand
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The intersection of flow cytometry with microfluidics and microfabrication.
A modern flow cytometer can analyze and sort particles on a one by one basis at rates of 50,000 particles per second. Flow cytometers can also measure as many as 17 channels of fluorescence, severalExpand
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A rapid multiplex assay for nucleic acid-based diagnostics.
We have developed a rapid (under 4 hours), multiplex, nucleic acid assay, adapted to a microsphere array detection platform. We call this assay multiplex oligonucleotide ligation-PCR (MOL-PCR).Expand
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Particle focusing in staged inertial microfluidic devices for flow cytometry.
Microfluidic inertial focusing has been demonstrated to be an effective method for passively positioning microparticles and cells without the assistance of sheath fluid. Because inertial focusingExpand
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An endogenous ouabain-like factor associated with hypertensive pregnant women.
Levels of a ouabain-like factor (OLF) were measured in amniotic fluid from 49 undigitalized third trimester pregnant women by means of its cross-reactivity in a digoxin RIA and its inhibition ofExpand
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Protein binding of endogenous digoxin-immunoactive factors in human serum and its variation with clinical condition.
  • R. Valdes, S. Graves
  • Chemistry, Medicine
  • The Journal of clinical endocrinology and…
  • 1 June 1985
We previously identified endogenous digoxin-like immunoactivity in serum from pregnant women, newborn infants, and patients in renal failure. This activity is due to an endogenous factor(s) thatExpand
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A flow cytometer for the measurement of Raman spectra
Multiparameter measurements in flow cytometry are limited by the broad emission spectra of fluorescent labels. By contrast, Raman spectra are notable for their narrow spectral features. To increaseExpand
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Analysis of low-abundance, low-molecular-weight serum proteins using mass spectrometry.
To detect diseases early in the general population, new diagnostic approaches are needed that have adequate sensitivity and specificity. Recent studies have used mass spectrometry to identify a serumExpand
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A prospective study of calciotropic hormones in pregnancy and post partum: reciprocal changes in serum intact parathyroid hormone and 1,25-dihydroxyvitamin D.
OBJECTIVE The purpose of this study was to examine the hormones regulating calcium homeostasis longitudinally in pregnancy and post partum. STUDY DESIGN Twenty-three women with normal pregnanciesExpand
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Inertial manipulation and transfer of microparticles across laminar fluid streams.
A general strategy for controlling particle movement across streams would enable new capabilities in single-cell analysis, solid-phase reaction control, and biophysics research. Transferring cellsExpand
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