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Performance comparison of benchtop high-throughput sequencing platforms
The performance of these instruments were compared by sequencing an isolate of Escherichia coli O104:H4, which caused an outbreak of food poisoning in Germany in 2011, and the MiSeq had the highest throughput per run and lowest error rates. Expand
Pathways of glutamate catabolism among Fusobacterium species.
The pathways of glutamate catabolism bore no relationship to the site of isolation of the fusobacteria tested but instead correlated with their chemotaxonomic properties, and F. varium, F. nucleatum and other species that have a lanthionine-based murein metabolized glutamate solely by the 2-oxoglutarate pathway. Expand
Mathematical Modelling of Mycobacterium tuberculosis VNTR Loci Estimates a Very Slow Mutation Rate for the Repeats
- A. Grant, C. Arnold, N. Thorne, S. Gharbia, A. Underwood
- Biology, Medicine
- Journal of Molecular Evolution
- 6 May 2008
It is shown that the repeats at a locus are likely to evolve by stepwise contraction or expansion in the number of repeats, which suggests that the VNTR profile of a strain of M. tuberculosis will be indicative of its clonal lineage and will be unlikely to vary in epidemiologically-related strains. Expand
Biochemical and chemical studies on strains designated Prevotella intermedia and proposal of a new pigmented species, Prevotella nigrescens sp. nov.
It is proposed that a new species, Prevotella nigrescens, should be created for the genetically distinct group of strains that hybridized with strain ATCC 33563T, which is designated the type strain of P. Nigrescens. Expand
Characterization of Prevotella intermedia and Prevotella nigrescens isolates from periodontic and endodontic infections.
The results show that P. intermedia and P. nigrescens may have different site specificities and surface properties and thus emphasize the need for accurate identification of these two species for the evaluation of their role in the pathogenesis of oral infections. Expand
Demonstration that 1-trans-epoxysuccinyl-L-leucylamido-(4-guanidino) butane (E-64) is one of the most effective low Mr inhibitors of trypsin-catalysed hydrolysis. Characterization by kinetic analysis…
- S. Sreedharan, C. Verma, +4 authors K. Brocklehurst
- Chemistry, Medicine
- The Biochemical journal
- 15 June 1996
1-trans-Epoxysuccinyl-L-leucylamido(4-guanidino)butane (E-64) was shown to inhibit beta-trypsin by a reversible competitive mechanism; this contrasts with the widely held view that E-64 is a… Expand
Biochemical and chemical analyses of black-pigmented gram-negative anaerobes.
Heterogeneity within Fusobacterium nucleatum, proposal of four subspecies
Fusobacterium nucleatum strains, isolated from man and animals, were shown to comprise four centres of variation within the species by using a variety of biochemical tests that indicated differences between the groups to warrant their placement into four subspecies. Expand
Fusobacterium nucleatum subsp. fusiforme subsp. nov. and Fusobacterium nucleatum subsp. animalis subsp. nov. as additional subspecies within Fusobacterium nucleatum.
This paper shows that the two remaining groups of strains within the species Fusobacterium nucleatum are distinct and formally propose that they should be recognized as F. nucleatum and F. polymorphum. Expand
Automated comparative sequence analysis by base-specific cleavage and mass spectrometry for nucleic acid-based microbial typing
- C. Honisch, Yong Chen, +6 authors S. Gharbia
- Biology, Medicine
- Proceedings of the National Academy of Sciences
- 19 June 2007
Comparison sequencing by MALDI-TOF MS for automated high-throughput microbial DNA sequence analysis shows interlaboratory reproducibility and allows for the exchange of mass spectrometric fingerprints to study the geographic spread of epidemic N. meningitidis strains or other microbes of clinical importance. Expand