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Identification, Expression, and Substrate Specificity of a Mammalian β-Carotene 15,15′-Dioxygenase*
We have identified from mouse the first mammalian β-carotene 15,15′-dioxygenase (β-CD), a crucial enzyme in development and metabolism that governs the de novo entry of vitamin A from plant-derivedExpand
A QTL on distal Chromosome 3 that influences the severity of light-induced damage to mouse photoreceptors
Finding light damage-modifying genes in the mouse may open avenues of study for understanding age-related macular degeneration and other retinal degenerations, since light exposures may contribute to the course of these diseases. Expand
Mutation of key residues of RPE65 abolishes its enzymatic role as isomerohydrolase in the visual cycle.
Findings establish a catalytic role, in conjunction with lecithin:retinol acyltransferase, for RPE65 in synthesis of 11-cis- retinol, and its identity as the isomerohydrolase. Expand
The DHHC domain: A new highly conserved cysteine-rich motif
A unique clone from a human pancreatic cDNA library was isolated and sequenced. Examination of the deduced polypeptide sequence of the clone showed a new form of cysteine-rich domain that included aExpand
Identification of beta‐carotene 15,15′‐monooxygenase as a peroxisome proliferator‐activated receptor target gene
PPAR is a key transcription factor for the transcriptional regulation of the Bcm gene, suggesting a broader function for PPARs in the regulation of carotenoid metabolism metabolism that is consistent with their established role in neutral lipid metabolism and transport. Expand
RPE65 Is an Iron(II)-dependent Isomerohydrolase in the Retinoid Visual Cycle*
Results indicate that RPE65 is an iron-dependent isomerohydrolase in the visual cycle and two specific iron-staining methods showed that purified R PE65 contains endogenous iron. Expand
RPE65, Visual Cycle Retinol Isomerase, Is Not Inherently 11-cis-specific
RPE65 is not inherently 11-cis-specific and can produce both 11- and 13- cis isomers, supporting a carbocation (or radical cation) mechanism for isomerization. Expand
Analysis of a human cDNA containing a tissue-specific alternatively spliced LIM domain.
RT-PCR and genomic sequence analyses indicate that expression of this gene product is subject to tissue-specific modulation by elimination of the LIM domain by alternative splicing in neural tissues. Expand
Key Role of Conserved Histidines in Recombinant Mouse β-Carotene 15,15′-Monooxygenase-1 Activity*
The in vitro and in vivo data showed that mutation of any of the four conserved histidines and Glu405 caused total loss of activity, and these residues are absolutely required for the catalytic mechanism of BCMO1. Expand
Laminin-induced retinoblastoma cell differentiation: possible involvement of a 100-kDa cell-surface laminin-binding protein.
This material, which the authors call LBM-100 (100-kDa laminin-binding molecule), may be a "differentiative" laminIn-binding protein through which lamin in influences gene expression and development independently of attachment. Expand