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Hypoxia-induced assembly of prolyl hydroxylase PHD3 into complexes: implications for its activity and susceptibility for degradation by the E3 ligase Siah2.
PHD1-3 (prolyl hydroxylases 1-3) catalyse the hydroxylation of HIF (hypoxia-inducible factor)-alpha subunit that triggers the substrate ubiquitination and subsequent degradation. The RING (reallyExpand
Stability of Homologue of Slimb F-box Protein Is Regulated by Availability of Its Substrate*
The homologue of Slimb (HOS) F-box protein is a receptor of the Skp1-Cullin1-F-box protein (SCFHOS) E3 ubiquitin ligase, which mediates ubiquitination and degradation of β-catenin and the inhibitorExpand
Autoinhibitory regulation of SCF-mediated ubiquitination by human cullin 1's C-terminal tail
SCF (Skp1·CUL1·F-box protein·ROC1) E3 ubiquitin ligase and Cdc34 E2-conjugating enzyme catalyze polyubiquitination in a precisely regulated fashion. Here, we describe biochemical evidence suggestingExpand
Proximity-induced activation of human Cdc34 through heterologous dimerization.
Cdc34 is an E2-conjugating enzyme required for catalyzing the polyubiquitination reaction mediated by the Skp1.CUL1.F-box (SCF) protein E3 ubiquitin (Ub) ligase. Here, we show that the activity ofExpand
Human Cdc34 Employs Distinct Sites To Coordinate Attachment of Ubiquitin to a Substrate and Assembly of Polyubiquitin Chains
ABSTRACT The Cdc34 E2 ubiquitin (Ub) conjugating enzyme catalyzes polyubiquitination of a substrate recruited by the Skp1-Cullin 1-F-box protein-ROC1 E3 Ub ligase. Using mutagenesis studies, we nowExpand
Functional Brachyury Binding Sites Establish a Temporal Read-out of Gene Expression in the Ciona Notochord
During notochord formation in chordate embryos, the transcription factor Brachyury employs different regulatory strategies to ensure the sequential activation of downstream genes and thereby theExpand
Temporal regulation of the muscle gene cascade by Macho1 and Tbx6 transcription factors in Ciona intestinalis
For over a century, muscle formation in the ascidian embryo has been representative of ‘mosaic’ development. The molecular basis of muscle-fate predetermination has been partly elucidated with theExpand
Temporal regulation of the muscle gene cascade by Macho1 and Tbx6 transcription factors in Ciona intestinalis
Introduction Ascidians, or tunicates, provide an essential genetic and evolutionary reference for studies of chordate development because of their well-characterized modes of development, theirExpand