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Distinct sequential cell behaviours direct primitive endoderm formation in the mouse blastocyst
TLDR
The events leading to PrE and EPI lineage segregation in the mouse are investigated by combining live imaging of embryos expressing a histone H2B-GFP fusion protein reporter under the control of Pdgfra regulatory elements with the analysis of lineage-specific markers. Expand
Primitive endoderm differentiates via a three-step mechanism involving Nanog and RTK signaling.
TLDR
A mosaic knockdown of Nanog is found to be sufficient to induce a PrE fate cell autonomously, and subsequent expression of Sox17 and Gata4--later markers of the PrE--depends on the presence of Fgf4 produced by Nanog-expressing cells. Expand
Downregulation of Par3 and aPKC function directs cells towards the ICM in the preimplantation mouse embryo
TLDR
It is shown that both Par3 and aPKC adopt a polarized localization from the 8-cell stage onwards and that manipulating their function re-directs cell positioning and consequently influences cell fate, illustrating a powerful means of manipulating gene expression in a specific clonal population of cells in the preimplantation embryo. Expand
Expression of multiple cytochrome p450 enzymes and multidrug resistance-associated transport proteins in human skin keratinocytes.
TLDR
Keratinocytes of the human skin express various transport-associated enzymes and detoxifying metabolic enzymes, and constitutive expression of multidrug resistance-associated proteins 1 and 3-6, and lung resistance protein in human epithelial keratinocytes is revealed. Expand
The evolution of class V POU domain transcription factors in vertebrates and their characterisation in a marsupial.
TLDR
The evolution of class V POU domain transcription factors is explored and it is shown that POU5F1 arose by gene duplication of pou2 early in the evolution of tetrapods and is not mammal-specific, as previously thought. Expand
Differential plasticity of epiblast and primitive endoderm precursors within the ICM of the early mouse embryo
TLDR
It is proposed that the early embryo environment restricts the fate choice of epiblast but not PrE precursors, thus ensuring the formation and preservation of the pluripotent foetal lineage. Expand
On the origin of POU5F1
TLDR
This study resolves the controversial evolutionary relationship between teleost pou2 and tetrapod POU2 and POU5F1, and shows that class V POU transcription factors have existed at least since the common ancestor of gnathostome vertebrates. Expand
Evolution of vertebrate interferon inducible transmembrane proteins
TLDR
This study characterised IFITMs in two distantly related marsupial species, the Australian tammar wallaby and the South American grey short-tailed opossum, and analysed the phylogeny of the IFITM family in vertebrates. Expand
Anatomy of a blastocyst: Cell behaviors driving cell fate choice and morphogenesis in the early mouse embryo
TLDR
An overview of the current understanding of the mechanisms governing these cell fate decisions ensuring proper lineage allocation and segregation, while at the same time providing the embryo with an inherent flexibility to adjust when perturbed is provided. Expand
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