• Publications
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Efficient TALEN-mediated gene knockout in livestock
TLDR
TALENs appear to represent a highly facile platform for the modification of livestock genomes for both biomedical and agricultural applications.
In vivo Genome Editing Using High Efficiency TALENs
TLDR
Improvements in artificial transcription activator-like effector nucleases (TALENs) provide a powerful new approach for targeted zebrafish genome editing and functional genomic applications and offer the potential to model genetic variation as well as to generate targeted conditional alleles.
Efficient nonmeiotic allele introgression in livestock using custom endonucleases
TLDR
It is demonstrated that genetic variants can be directly introgressed into livestock genomes using a modified transcription activator-like effector nuclease system and expanded the livestock gene editing toolbox to include TALEN and clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9-stimulated homology-directed repair (HDR).
Genome edited sheep and cattle
TLDR
Zygote injection of TALEN mRNA can also produce gene-edited cattle and sheep and provides a practical alternative to somatic cell nuclear transfer for gene knockout or introgression of desirable alleles into a target breed/genetic line.
Genetic immunization of rainbow trout (Oncorhynchus mykiss) against infectious hematopoietic necrosis virus.
TLDR
These studies show that DNA vaccination will protect rainbow trout against the lethal effects of IHNV infection.
Live pigs produced from genome edited zygotes
TLDR
It is demonstrated for the first time that both TALEN and ZFN injected directly into pig zygotes can produce live genome edited pigs.
Bicarbonate‐dependent chloride transport drives fluid secretion by the human airway epithelial cell line Calu‐3
TLDR
It is shown that fluid secretion requires chloride, bicarbonate and sodium, that chloride is the predominant anion in Calu‐3 secretions, and that a large fraction of the basolateral chloride loading during cAMP stimulation occurs by Cl−/HCO3− exchange.
Porcine gene discovery by normalized cDNA-library sequencing and EST cluster assembly
TLDR
Cluster analysis revealed that within-library redundancy is low, and comparison of all porcine ESTs with the human database suggests that the sequences from these two libraries represent portions of a significant number of independent pig genes.
Construction and characterization of a new bovine bacterial artificial chromosome library with 10 genome-equivalent coverage
TLDR
This research presents a novel and scalable approach called “cell reprograming” that allows for real-time, 3D analysis of the response of the immune systems of animals to antibiotics.
Two Zebrafish eIF4E Family Members Are Differentially Expressed and Functionally Divergent*
Eukaryotic translation initiation factor 4E (eIF4E) is an essential component of the translational machinery that binds m7GTP and mediates the recruitment of capped mRNAs by the small ribosomal
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