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Dynamic Ligand-induced Conformational Rearrangements in P-glycoprotein as Probed by Fluorescence Resonance Energy Transfer Spectroscopy*
TLDR
The two nucleotide binding domains are found to be in close association during the catalytic cycle as determined by fluorescence spectroscopy, suggesting the presence of at least three major conformations of the NBDs during catalysis.
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36° step size of proton‐driven c‐ring rotation in FoF1‐ATP synthase
TLDR
Using single‐molecule fluorescence resonance energy transfer, this work provides the first experimental determination of a 36° sequential stepping mode of the c‐ring during ATP synthesis.
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YidC-driven membrane insertion of single fluorescent Pf3 coat proteins.
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Elastic deformations of the rotary double motor of single F(o)F(1)-ATP synthases detected in real time by Förster resonance energy transfer.
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Monitoring the rotary motors of single FoF1-ATP synthase by synchronized multi channel TCSPC
TLDR
The action mode of bactericidal drugs, i.e. inhibitors of FoF1-ATP synthase like aurovertin, could be investigated by the time resolved single-molecule FRET approach.
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Real Time Observation of Single Membrane Protein Insertion Events by the Escherichia coli Insertase YidC
TLDR
This work focuses on a minimal system, the membrane insertase YidC of Escherichia coli that inserts small proteins into the cytoplasmic membrane, and reports on the translocation of the N-terminal domain of Pf3 coat protein across the membrane in real time.
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Simultaneous monitoring of the two coupled motors of a single FoF1-ATP synthase by three-color FRET using duty cycle-optimized triple-ALEX
TLDR
To reduce photophysical artifacts due to spectral fluctuations of the single fluorophores, a duty cycle-optimized alternating three-laser scheme (DCO-ALEX) has been developed and simultaneous observation of the stepsizes for both motors allows the detection of reversible elastic deformations between the rotor parts of Fo and F1.
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Three-color Förster resonance energy transfer within single F₀F₁-ATP synthases: monitoring elastic deformations of the rotary double motor in real time.
TLDR
This work presents a new single-molecule FRET approach to observe both rotary motors simultaneously in a single F(O)F(1)-ATP synthase at work, and labels this enzyme with three fluorophores, specifically at the stator part and at the two rotors.
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Analyzing conformational dynamics of single P-glycoprotein transporters by Förster resonance energy transfer using hidden Markov models.
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Legionella effector AnkX displaces the switch II region for Rab1b phosphocholination
TLDR
The combination of chemical biology and structural analysis reveals the enzymatic mechanism of AnkX and the family of filamentation induced by cyclic adenosine monophosphate (FIC) proteins.
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