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In vitro activation of ammonia monooxygenase from Nitrosomonas europaea by copper
TLDR
It is proposed that loss of AMO activity upon lysis of N. europaea results from the loss of copper from AMO, generating a catalytically inactive, yet stable and activable, form of the enzyme. Expand
Microbial metabolism of aliphatic alkenes.
  • S. Ensign
  • Chemistry, Medicine
  • Biochemistry
  • 22 May 2001
Evidence that a Linear Megaplasmid Encodes Enzymes of Aliphatic Alkene and Epoxide Metabolism and Coenzyme M (2-Mercaptoethanesulfonate) Biosynthesis in XanthobacterStrain Py2
TLDR
It is proposed that the propylene-positive phenotype of Xanthobacter strain Py2 is dependent on the selective maintenance of a linear megaplasmid containing the genes for the key enzymes of alkene oxidation, epoxide carboxylation, and CoM biosynthesis. Expand
Genetic and physiological characterization of the Rhodospirillum rubrum carbon monoxide dehydrogenase system
TLDR
A 3.7-kb DNA region encoding part of the Rhodospirillum rubrum CO oxidation (coo) system was identified by using oligonucleotide probes and it is posit that cooH and cooFS are not cotranscribed. Expand
Biochemical, Molecular, and Genetic Analyses of the Acetone Carboxylases from Xanthobacter autotrophicus Strain Py2 and Rhodobacter capsulatus Strain B10
TLDR
A conserved strategy of ATP-dependent acetoneCarboxylation and the involvement of transcriptional enhancers in acetone carboxylase gene expression in gram-negative acetone-utilizing bacteria is revealed. Expand
Aliphatic and chlorinated alkenes and epoxides as inducers of alkene monooxygenase and epoxidase activities in Xanthobacter strain Py2
  • S. Ensign
  • Medicine, Chemistry
  • Applied and environmental microbiology
  • 1 January 1996
TLDR
The inducible nature of the alkene oxidation system of Xanthobacter strain Py2 has been investigated and it was revealed that the 43-, 53-, and 57-kDa proteins, as well as two additional polypeptides, were newly synthesized upon exposure of cells to propylene or propylene oxide. Expand
Cometabolic degradation of chlorinated alkenes by alkene monooxygenase in a propylene-grown Xanthobacter strain
TLDR
No toxic effects were seen when cells were incubated with chlorinated alkenes anaerobically, indicating that the product(s) of chlorinated alkene oxidation mediates toxicity. Expand
Alkene Monooxygenase from Xanthobacter Strain Py2
  • F. Small, S. Ensign
  • Chemistry, Medicine
  • The Journal of Biological Chemistry
  • 3 October 1997
TLDR
The biochemical properties of alkene monooxygenase described in this study suggest that this enzyme combines elements of both the well-characterized aromatic dioxygenase (two-component electron transfer scheme) and methane mono oxygenase (small regulatory protein and diiron oxygenase) multicomponent enzyme systems. Expand
Purification and characterization of acetone carboxylase from Xanthobacter strain Py2.
  • M. Sluis, S. Ensign
  • Chemistry, Medicine
  • Proceedings of the National Academy of Sciences…
  • 5 August 1997
TLDR
Molecular properties of the first bacterial acetone-metabolizing enzyme to be isolated are revealed and a novel mechanism of acetone carboxylation coupled to ATP hydrolysis and AMP and inorganic phosphate formation is suggested. Expand
A role for coenzyme M (2-mercaptoethanesulfonic acid) in a bacterial pathway of aliphatic epoxide carboxylation.
TLDR
A newfound versatility for coenzyme M as a carrier and activator of alkyl groups longer in chain-length than methane, a function for coENzyme M in a catabolic pathway of hydrocarbon oxidation, and the presence of coen enzyme M in the bacterial domain of the phylogenetic tree are evince. Expand
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