In vitro activation of ammonia monooxygenase from Nitrosomonas europaea by copper
It is proposed that loss of AMO activity upon lysis of N. europaea results from the loss of copper from AMO, generating a catalytically inactive, yet stable and activable, form of the enzyme.
Microbial metabolism of aliphatic alkenes.
- S. Ensign
- BiologyBiochemistry
- 22 May 2001
Evidence that a Linear Megaplasmid Encodes Enzymes of Aliphatic Alkene and Epoxide Metabolism and Coenzyme M (2-Mercaptoethanesulfonate) Biosynthesis in XanthobacterStrain Py2
- J. G. Krum, S. Ensign
- Biology, ChemistryJournal of Bacteriology
- 1 April 2001
It is proposed that the propylene-positive phenotype of Xanthobacter strain Py2 is dependent on the selective maintenance of a linear megaplasmid containing the genes for the key enzymes of alkene oxidation, epoxide carboxylation, and CoM biosynthesis.
Cometabolic degradation of chlorinated alkenes by alkene monooxygenase in a propylene-grown Xanthobacter strain
No toxic effects were seen when cells were incubated with chlorinated alkenes anaerobically, indicating that the product(s) of chlorinated alkene oxidation mediates toxicity.
Biochemical, Molecular, and Genetic Analyses of the Acetone Carboxylases from Xanthobacter autotrophicus Strain Py2 and Rhodobacter capsulatus Strain B10
- M. Sluis, R. A. Larsen, J. G. Krum, Ruth Anderson, W. Metcalf, S. Ensign
- BiologyJournal of Bacteriology
- 1 June 2002
A conserved strategy of ATP-dependent acetoneCarboxylation and the involvement of transcriptional enhancers in acetone carboxylase gene expression in gram-negative acetone-utilizing bacteria is revealed.
Aliphatic and chlorinated alkenes and epoxides as inducers of alkene monooxygenase and epoxidase activities in Xanthobacter strain Py2
- S. Ensign
- ChemistryApplied and Environmental Microbiology
- 1 January 1996
The inducible nature of the alkene oxidation system of Xanthobacter strain Py2 has been investigated and it was revealed that the 43-, 53-, and 57-kDa proteins, as well as two additional polypeptides, were newly synthesized upon exposure of cells to propylene or propylene oxide.
Genetic and physiological characterization of the Rhodospirillum rubrum carbon monoxide dehydrogenase system
- R. Kerby, S. S. Hong, S. Ensign, L. J. Coppoc, P. Ludden, G. Roberts
- BiologyJournal of Bacteriology
- 1 August 1992
A 3.7-kb DNA region encoding part of the Rhodospirillum rubrum CO oxidation (coo) system was identified by using oligonucleotide probes and it is posit that cooH and cooFS are not cotranscribed.
Alkene Monooxygenase from Xanthobacter Strain Py2
- F. J. Small, S. Ensign
- Biology, ChemistryJournal of Biological Chemistry
- 3 October 1997
The biochemical properties of alkene monooxygenase described in this study suggest that this enzyme combines elements of both the well-characterized aromatic dioxygenase (two-component electron transfer scheme) and methane mono oxygenase (small regulatory protein and diiron oxygenase) multicomponent enzyme systems.
A role for coenzyme M (2-mercaptoethanesulfonic acid) in a bacterial pathway of aliphatic epoxide carboxylation.
- J. Allen, D. D. Clark, J. G. Krum, S. Ensign
- Chemistry, BiologyProceedings of the National Academy of Sciences…
- 20 July 1999
A newfound versatility for coenzyme M as a carrier and activator of alkyl groups longer in chain-length than methane, a function for coENzyme M in a catabolic pathway of hydrocarbon oxidation, and the presence of coen enzyme M in the bacterial domain of the phylogenetic tree are evince.
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