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A new vital stain for visualizing vacuolar membrane dynamics and endocytosis in yeast
  • T. Vida, S. Emr
  • Biology, Medicine
  • The Journal of cell biology
  • 1 March 1995
TLDR
It is shown that FM 4-64 is a new vital stain for the vacuolar membrane, a marker for endocytic intermediates, and a fluor for detecting endosome to vacuole membrane transport in vitro. Expand
Ubiquitin-Dependent Sorting into the Multivesicular Body Pathway Requires the Function of a Conserved Endosomal Protein Sorting Complex, ESCRT-I
TLDR
It is demonstrated that ubiquitination serves as a signal for sorting into the multivesicular body pathway and proposed that ESCRT-I represents a conserved component of the endosomal sorting machinery that functions in both yeast and mammalian cells to couple ubiquitin modification to protein sorting and receptor downregulation in the MVB pathway. Expand
Receptor downregulation and multivesicular-body sorting
The sorting of proteins into the inner vesicles of multivesicular bodies is required for many key cellular processes, which range from the downregulation of activated signalling receptors to theExpand
A Membrane Coat Complex Essential for Endosome-to-Golgi Retrograde Transport in Yeast
TLDR
It is shown that Vps35p and Vps29p interact and form part of a multimeric membrane-associated complex that also contains Vps26p, Vps17p, and VPS5p, which suggests an important general role for this complex in endosome-to-Golgi retrieval. Expand
Escrt-III: an endosome-associated heterooligomeric protein complex required for mvb sorting.
TLDR
There is evidence for a role for ESCRT-III in sorting and/or concentration of MVB cargoes in the multivesicular body (MVB) pathway to the lysosomal/vacuolar lumen. Expand
Autophagy as a regulated pathway of cellular degradation.
TLDR
The core protein machinery that is necessary to drive formation and consumption of intermediates in the macroautophagy pathway includes a ubiquitin-like protein conjugation system and a protein complex that directs membrane docking and fusion at the lysosome or vacuole. Expand
The ESCRT pathway.
Multivesicular bodies (MVBs) deliver cargo destined for degradation to the vacuole or lysosome. The ESCRT (endosomal sorting complex required for transport) pathway is a key mediator of MVBExpand
The Vps4p AAA ATPase regulates membrane association of a Vps protein complex required for normal endosome function
TLDR
Together, the data suggest that the Vps4 ATPase catalyzes the release of an endosomal membrane‐associated class E protein complex(es) required for normal morphology and sorting activity of the endosome. Expand
Endosome-associated complex, ESCRT-II, recruits transport machinery for protein sorting at the multivesicular body.
TLDR
This study characterizes ESCRT-II, a soluble approximately 155 kDa protein complex formed by the class E Vps proteins Vps22, Vps25, and Vps36, and proposes that the ESCRT complexes perform a coordinated cascade of events to select and sort MVB cargoes for delivery to the lumen of the vacuole/lysosome. Expand
New Component of the Vacuolar Class C-Vps Complex Couples Nucleotide Exchange on the Ypt7 Gtpase to Snare-Dependent Docking and Fusion
TLDR
The class C-Vps complex directs multiple reactions during the docking and fusion of vesicles with the vacuole, each of which contributes to the overall specificity and efficiency of this transport process. Expand
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