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[Purification and properties of chitinase from Enterobacter aerogenes].
A bacterium producing chitinase was isolated from the dead body of Gymephorap ruoergensis and purified by means of ammonium sulfate precipitation, DEAE-cellulose column chromatography, and Sephadex G-100 column gel filtration, and showed homogeneity on the native polyacrylamide gel electrophoresis. Expand
[N-terminal analysis and antibody preparation of insecticidal protein form Pseudomonas pseudoalcaligenes].
  • S. Ding, Z. Yang
  • Chemistry, Medicine
  • Wei sheng wu xue bao = Acta microbiologica Sinica
  • 1 October 2001
Western blot analysis revealed that the antibody was spectific to 26 kD insecticidal protein, and did not cross-react with other proteins produced by the bacterium, suggesting that a specific antibody with high titer was obtained and could be used for further investigations of the gene cloning and expression of insecticidalprotein. Expand
[Purification and properties of glutamate dehydrogenase from Pseudomonas pseudoalcaligenes].
Glutamate dehydrogenase was purified from the crude extract of Pseudomonas pseudoal-caligenes and showed considerable loss of activity upon freezing. Expand