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Bacterial diversity in a deep-subsurface clay environment
TLDR
The presence of bacteria in a deep clay sediment was analyzed in a 20-m-long core horizontally drilled from a mine gallery at a depth of 224 m in the Boom clay formation (Mol, Belgium) to demonstrate the difficulty of analyzing samples that contain only a few poorly active bacteria as it is difficult to avoid a small contamination by active bacteria during sampling. Expand
Microbial Community Analysis of Opalinus Clay Drill Core Samples from the Mont Terri Underground Research Laboratory, Switzerland
TLDR
The combined results of this study suggest that unperturbed Opalinus Clay appears to contain only a small viable microbial community, which is probably metabolically almost inactive (dormant), due to space and water restrictions. Expand
Comparison of phenotypical and molecular methods for the identification of bacterial strains isolated from a deep subsurface environment
TLDR
Data clearly showed that a phylogenetic analysis of small subunit rDNA sequences is more efficient than classical phenotypic methods for the identification of bacterial strains freshly isolated from a natural environment, because occurrences of misidentification are very much decreased by this method. Expand
Comparison of phenotypical and molecular methods for the identification of bacterial strains isolated from a deep subsurface environment
TLDR
This work was supported by the CNRS and by grants of the European Community and ANDRA as part of the project Archimede-Argiles and of the Direction de la Recherche and des Etudes Doctorales. Expand
Occurrence and identification of microorganisms in compacted clay-based buffer material designed for use in a nuclear fuel waste disposal vault.
TLDR
The results suggest that a nuclear fuel waste buffer will be populated by active microorganisms only if the moisture content is above a value where free water is available for active life. Expand
Microstructural characterization of carbon steel corrosion in clay borehole water under anoxic and transient acidic conditions
Abstract The corrosion interface of low-alloy carbon steel (C-steel) coupons (P235, ferrite-pearlitic with a columnar microstructure) reacted in clay porewater seeping in a test chamber in situ inExpand
Corrosion at the carbon steelclay borehole water and gas interfaces at 85 °C under anoxic and transient acidic conditions
In order to understand the corrosion processes of low-alloyed carbon steel (C-steel) materials in contact with porewater and/or gas in clay environments under reducing conditions at 85 °C, an in situExpand
Corrosion at the carbon steel-clay borehole water interface under anoxic alkaline and fluctuating temperature conditions
Abstract Coupons of carbon steel were corroded in situ in anoxic clay porewater under slightly alkaline conditions. Sample damage was less than 1 μm for 9 months at 85 °C only, and corrosionExpand
Laboratory simulation of an oxidizing perturbation in a deep granite environment
Abstract An experiment designed to study oxidizing perturbations in deep crystalline rock, a potential host for nuclear waste disposal, was conducted. This experiment simulated a fracture surface inExpand
Thermanaeromonas burensis sp. nov., a thermophilic anaerobe isolated from a subterranean clay environment.
TLDR
A strictly anaerobic, thermophilic and halotolerant strain, isolated from the seepage water collected in a metal biocorrosion test at a depth of 490 m, in a subterranean Callovo-Oxfordian clay formation in northern France represents a novel species of the genus Thermanaeromonas. Expand
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