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Deep sequencing analysis of RNAs from a grapevine showing Syrah decline symptoms reveals a multiple virus infection that includes a novel virus.
In a search for viruses associated with decline symptoms of Syrah grapevines, we have undertaken an analysis of total plant RNA sequences using Life Sciences 454 high-throughput sequencing. 67.5Expand
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Deep sequencing evidence from single grapevine plants reveals a virome dominated by mycoviruses
We have characterized the virome in single grapevines by 454 high-throughput sequencing of double-stranded RNA recovered from the vine stem. The analysis revealed a substantial set of sequencesExpand
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Genomic and biological analysis of Grapevine leafroll-associated virus 7 reveals a possible new genus within the family Closteroviridae.
Deep sequencing analysis of an asymptomatic grapevine revealed a virome containing five RNA viruses and a viroid. Of these, Grapevine leafroll-associated virus 7 (GLRaV-7), an unassignedExpand
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Gene from a novel plant virus satellite from grapevine identifies a viral satellite lineage
We have identified the genome of a novel viral satellite in deep sequence analysis of double-stranded RNA from grapevine. The genome was 1,060 bases in length, and encoded two open reading frames.Expand
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Comparison of Next-Generation Sequencing Versus Biological Indexing for the Optimal Detection of Viral Pathogens in Grapevine.
A bioassay is routinely used to determine the viral phytosanitary status of commercial grapevine propagation material in many countries around the world. That test is based on the symptoms developedExpand
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Development of a Sensitive Colorimetric-PCR Assay for Detection of Viruses in Woody Plants.
Diagnostic methods employing the polymerase chain reaction (PCR) provide the most sensitive means currently available for detecting viruses in woody plants. A new technique has been tested that doesExpand
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Characterization of latent viral infection of olive trees in the National Clonal Germplasm Repository in California.
The oil- and table olive industry in California is growing rapidly. Propagation material is being requested in ever-larger quantities from the USDA National Clonal Germplasm Repository at theExpand
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CHARACTERIZATION OF A FIFTH VITIVIRUS IN GRAPEVINE
A virus species provisionally named Grapevine virus F (GVF) was found in grapevine by deep sequencing. GVF is a vitivirus distinctly different from the other four members of this genus, as shown byExpand
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Synergy between grapevine vitiviruses and grapevine leafroll viruses
An interactive relationship between vitiviruses and grapevine leafroll viruses was characterized in grapevine. Grapevine viruses A and B (GVA and GVB) were found more frequently in the presence ofExpand
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Polymerase Chain Reaction Methods for the Detection of Grapevine Viruses and Viroids
The need to diagnose and manage viral pathogens that have been accumulating in grapevines across most of recorded history has become a central focus of modern viticulture. In recent times, theExpand
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