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Use of the flagellar H7 gene as a target in multiplex PCR assays and improved specificity in identification of enterohemorrhagic Escherichia coli strains.
TLDR
The multiplex PCR assays described allow for the specific identification of E. coli O157:H7 or NM and other EHEC strains and vt, eaeA, and fliC PCR products were observed with DNAs from the majority of EH EC strains. Expand
Structural insight into the ligand specificity of a thermostable family 51 arabinofuranosidase, Araf51, from Clostridium thermocellum.
TLDR
The paucity of direct hydrogen bonds with the aglycone moiety and the flexible conformation adopted by Trp(178), which stacks against the sugar at the +1 subsite, provide a structural explanation for the plasticity in substrate specificity displayed by the clostridial arabinofuranosidase. Expand
Specific identification of Escherichia coli O157:H7 using a multiplex PCR assay.
TLDR
A PCR assay was devised which is highly specific for E. coli O157:H7 and other EHEC using polymerase chain reaction (PCR) assays which target vt and eae genes and a 5′ conserved area of the eae gene. Expand
Structural insight into the ligand specificity of a thermostable family 51 arabinofuranosidase , Araf 51 , from Clostridium thermocellum
The digestion of the plant cell wall requires the concerted action of a diverse repertoire of enzyme activities. An important component of these hydrolase consortia are arabinofuranosidases, whichExpand
Microbiological verification of the control of the processes of dressing, cooling and processing of beef carcasses at a high line-speed abattoir
Abstract Methods were described, and are offered to inspection agencies, to verify the microbiological adequacy of the processes of dressing, chilling and fabricating beef carcasses. The isolationExpand