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Developing photoactive affinity probes for proteomic profiling: hydroxamate-based probes for metalloproteases.
A complimentary, affinity-based labeling approach to profile enzymes which do not possess covalently bound substrate intermediates is presented, making the approach useful for potential in situ screening of metalloprotease inhibitors. Expand
Strategies for site-specific protein biotinylation using in vitro, in vivo and cell-free systems: toward functional protein arrays
These methods were designed to maintain the integrity and activity of proteins in a microarray format, which potentially allows simultaneous functional assays of thousands of proteins. Expand
Recent developments in microarray-based enzyme assays: from functional annotation to substrate/inhibitor fingerprinting
This work reviews recent developments in the fabrication of various types of microarray technologies (protein-, peptide- and small-molecule-based microarrays) and their applications in high-throughput characterizations of enzymes. Expand
Expanding the chemical biologist's tool kit: chemical labelling strategies and its applications.
The various strategies available to "chemically" tag proteins are summarized and a comparative analysis their advantages and disadvantages are provided to highlight the many creative applications of such methodologies. Expand
Developing novel activity-based fluorescent probes that target different classes of proteases.
The design and synthesis of a group of novel activity-based probes that target different protease sub-classes based on their substrate specificities, rather than their enzymatic mechanisms are reported. Expand
Role of FK506 binding proteins in neurodegenerative disorders.
This review focuses on the roles of F KBPs in neurodegenerative disorders with an emphasis on the cellular mechanisms responsible for their neuroprotective and neurotrophic activities and the structural features of FKBPs and the mode of action of NILs. Expand
An affinity-based probe for the proteomic profiling of aspartic proteases
We have developed an affinity-based probe for the proteomic profiling of aspartic proteases. Our probe was shown to be selective towards aspartic proteases over other proteins. It was also shown thatExpand
Site-specific covalent labeling of proteins inside live cells using small molecule probes.
A small molecule-based approach that exploits the unique reactivity between the cysteine residue at the N-terminus of a target protein and cell-permeable, thioester-based small molecule probes resulting in site-specific, covalent tagging of proteins. Expand
Expanding the Chemical Biologists Tool Kit: Chemical Labelling Strategies and Its Applications
Methods that allow visualisation of proteins in living systems, in real time have been key to our understanding of the molecular underpinnings of life. Although the use of genetically encoded fusionsExpand
Advanced analytical tools in proteomics.
Recent advances in the bioassay development have provided sensitive and selective tools for high-throughput identification and characterizations of enzymes, and the recently developed bioimaging techniques to visualize and quantify proteins in living cells are discussed. Expand