• Publications
  • Influence
A tracked approach for automated NMR assignments in proteins (TATAPRO)
A novel automated approach for the sequence specific NMR assignments of 1HN, 13Cα, 13Cβ, 13C′/1Hα and 15N spins in proteins, using triple resonance experimental data, is presented. The algorithm,Expand
  • 108
  • 4
  • PDF
Regulation of Estrogen Receptor α N-Terminus Conformation and Function by Peptidyl Prolyl Isomerase Pin1
ABSTRACT Estrogen receptor alpha (ERα), a key driver of growth in the majority of breast cancers, contains an unstructured transactivation domain (AF1) in its N terminus that is a convergence pointExpand
  • 61
  • 3
  • PDF
Hydrogen exchange during cell-free incorporation of deuterated amino acids and an approach to its inhibition
Perdeuteration, selective deuteration, and stereo array isotope labeling (SAIL) are valuable strategies for NMR studies of larger proteins and membrane proteins. To minimize scrambling of the label,Expand
  • 19
  • 2
Backbone dynamics of free barnase and its complex with barstar determined by 15N NMR relaxation study
Backbone dynamics of uniformly 15N-labeled free barnase and its complex with unlabelled barstar have been studied at 40 °C, pH 6.6, using 15N relaxation data obtained from proton-detected 2D {1H}-15NExpand
  • 22
  • 2
  • PDF
NMR derived solution structure of an EF-hand calcium-binding protein from Entamoeba Histolytica.
We present the three-dimensional (3D) solution structure of a calcium-binding protein from Entamoeba histolytica (EhCaBP), an etiologic agent of amoebiasis affecting millions worldwide. EhCaBP is aExpand
  • 32
  • 1
  • PDF
Recombinant hemoglobin βG83C‐F41Y
We have engineered a stable octameric hemoglobin (Hb) of molecular mass 129 kDa, a dimer of recombinant hemoglobin (rHb βG83C‐F41Y) tetramers joined by disulfide bonds at the β83 position. One of theExpand
  • 15
  • 1
A comparative NMR study of the polypeptide backbone dynamics of hemoglobin in the deoxy and carbonmonoxy forms.
Model-free-based NMR dynamics studies have been undertaken for polypeptide backbone amide N-H bond vectors for both the deoxy and carbonmonoxy forms of chain-specific, isotopically (15N and 2H)Expand
  • 24
  • PDF
Conserved themes in target recognition by the PAH1 and PAH2 domains of the Sin3 transcriptional corepressor.
The recruitment of chromatin-modifying coregulator complexes by transcription factors to specific sites of the genome constitutes an important step in many eukaryotic transcriptional regulatoryExpand
  • 36
Insights into the solution structure of human deoxyhemoglobin in the absence and presence of an allosteric effector.
We present a nuclear magnetic resonance (NMR) study in solution of the structures of human normal hemoglobin (Hb A) in the deoxy or unligated form in the absence and presence of an allostericExpand
  • 27
Dynamics of allostery in hemoglobin: roles of the penultimate tyrosine H bonds.
The tyrosine residues adjacent to the C termini of the hemoglobin (Hb) subunits, alphaY140 and betaY145, are expected to play important structural roles, because the C termini are the loci of T-stateExpand
  • 26