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Molecular biological access to the chemistry of unknown soil microbes: a new frontier for natural products.
Cultured soil microorganisms have provided a rich source of natural-product chemistry. Because only a tiny fraction of soil microbes from soil are readily cultured, soil might be the greatestExpand
Cloning the Soil Metagenome: a Strategy for Accessing the Genetic and Functional Diversity of Uncultured Microorganisms
ABSTRACT Recent progress in molecular microbial ecology has revealed that traditional culturing methods fail to represent the scope of microbial diversity in nature, since only a small proportion ofExpand
Isolation of Antibiotics Turbomycin A and B from a Metagenomic Library of Soil Microbial DNA
ABSTRACT To access the genetic and biochemical potential of soil microorganisms by culture-independent methods, a 24,546-member library in Escherichia coli with DNA extracted directly from soil hadExpand
Minimum Information about a Biosynthetic Gene cluster.
A wide variety of enzymatic pathways that produce specialized metabolites in bacteria, fungi and plants are known to be encoded in biosynthetic gene clusters. Information about these clusters,Expand
Expanding Small-Molecule Functional Metagenomics through Parallel Screening of Broad-Host-Range Cosmid Environmental DNA Libraries in Diverse Proteobacteria
ABSTRACT The small-molecule biosynthetic diversity encoded within the genomes of uncultured bacteria is an attractive target for the discovery of natural products using functional metagenomics.Expand
Construction of soil environmental DNA cosmid libraries and screening for clones that produce biologically active small molecules
  • S. Brady
  • Biology, Medicine
  • Nature Protocols
  • 1 May 2007
Culture-independent studies on environmental samples indicate that most bacteria are not readily cultured in the laboratory. The small fraction of bacteria that have been successfully cultured fromExpand
Malleilactone, a polyketide synthase-derived virulence factor encoded by the cryptic secondary metabolome of Burkholderia pseudomallei group pathogens.
Sequenced bacterial genomes are routinely found to contain gene clusters that are predicted to encode metabolites not seen in fermentation-based studies. Pseudomallei group Burkholderia are emergingExpand
Cloning and heterologous expression of a natural product biosynthetic gene cluster from eDNA.
[reaction: see text] To study the natural products produced by uncultured microorganisms, an environmental DNA (eDNA) cosmid library was constructed and screened for the heterologous production ofExpand
Cloning and heterologous expression of isocyanide biosynthetic genes from environmental DNA.
Cultured soil bacteria have been an important source of biologically active, naturally occurring small molecules. Uncultured soil bacteria, which outnumber their cultured counterparts by at least twoExpand
Fluostatins produced by the heterologous expression of a TAR reassembled environmental DNA derived type II PKS gene cluster.
Culture independent approaches for accessing small molecules produced by uncultured bacteria are often hampered by the inability to easily clone environmental DNA (eDNA) fragments large enough toExpand