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Nitric oxide is required for, and promotes auxin-mediated activation of, cell division and embryogenic cell formation but does not influence cell cycle progression in alfalfa cell cultures.
It is now well established that nitric oxide (NO) serves as a signaling molecule in plant cells. In this paper experimental data are presented which indicate that NO can stimulate the activation ofExpand
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Immunolocalization of a novel annexin-like protein encoded by a stress and abscisic acid responsive gene in alfalfa.
We report here on the isolation and characterization of a full-length cDNA clone from alfalfa termed AnnMs2 encoding a 333 amino acid long polypeptide that shows 32-37% sequence identity with bothExpand
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Doubled Haploid Development and PCR-analysis of F1 Hybrid Derived DH-R2 Paprika (Capsicum annuum L.) Lines
Summary Anther derived spontaneous doubled haploid (DH-R 1 ) plants were developed from an intercultivar F 1 hybrid (Holland) of ‹blocky› type pepper cv. ‹Mazurka› ( Capsicum annuum L. var. annuum ,Expand
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Characterization of a family of Arabidopsis receptor-like cytoplasmic kinases (RLCK class VI)
The receptor-like cytoplasmic protein kinases (RLCKs) are plant-specific proteins encoded by almost 200 genes in the Arabidopsis genome. Despite of their high number, the available information on theExpand
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Characterization of Agrobacterium tumefaciens strains isolated from grapevine
Grapevine crown gall is predominantly induced by Agrobacterium vitis , but rarely, Agrobacterium tumefaciens may also cause this disease. We have collected 18 grapevine isolates of A. tumefaciensExpand
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Direct measurement of torque in an optical trap and its application to double-strand DNA.
We present a method that offers the possibility to directly apply and measure torque on particles in an optical trap. It can be used to rotationally manipulate biopolymers attached to appropriateExpand
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Production of transgenic maize plants by direct DNA uptake into embryogenic protoplasts
Abstract Fertile transgenic maize plants were regenerated after direct transfer of a chimeric gene into maize protoplasts. Plasmid DNA containing mutant dihydrofolate reductase (DHFR) mouse gene,Expand
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Hyperosmotic stress induces Axl activation and cleavage in cerebral endothelial cells
Because of the relative impermeability of the blood‐brain barrier (BBB), many drugs are unable to reach the CNS in therapeutically relevant concentration. One method to deliver drugs to the CNS isExpand
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The chl a fluorescence intensity is remarkably insensitive to changes in the chlorophyll content of the leaf as long as the chl a/b ratio remains unaffected.
The effects of changes in the chlorophyll (chl) content on the kinetics of the OJIP fluorescence transient were studied using two different approaches. An extensive chl loss (up to 5-fold decrease)Expand
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Identification and characterization of genes associated with the induction of embryogenic competence in leaf-protoplast-derived alfalfa cells.
Alfalfa leaf protoplast-derived cells can develop into somatic embryos depending on the concentration of 2,4-dichlorophenoxyacetic acid (2,4-D) in the initial culture medium. In order to reveal geneExpand
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