• Publications
  • Influence
Arabidopsis PEROXIN11c-e, FISSION1b, and DYNAMIN-RELATED PROTEIN3A Cooperate in Cell Cycle–Associated Replication of Peroxisomes[W]
TLDR
Data from bimolecular fluorescence complementation experiments with Arabidopsis thaliana suspension cells support a model for the replication of preexisting peroxisomes wherein PEX11c, Pex11d, and P EX11e act cooperatively during G2 to promote perox isome elongation and recruitment of FIS1b to the peroxISome membrane. Expand
Re-examining Alveolate Evolution Using Multiple Protein Molecular Phylogenies
TLDR
Six genes from the non-photosynthetic dinoflagellate, Crypthecodinium cohnii: actin, beta-tubulin, hSp70, BiP, hsp90, and mitochondrial hsp10 were found to be useful for intra-alveolate phylogeny, and trees were inferred from these genes individually and in combination. Expand
Changes in fatty acid profiles of thermo-intolerant and thermo-tolerant marine diatoms during temperature stress
TLDR
The hypothesis that a particular temperature, and not treatment duration, has the greater effect on changes in fatty acid composition and degree of fatty acid saturation is supported. Expand
A New Class of Transcription Initiation Factors, Intermediate between TATA Box-binding Proteins (TBPs) and TBP-like Factors (TLFs), Is Present in the Marine Unicellular Organism, the Dinoflagellate
TLDR
This is the first description of a TBP-like protein in a unicellular organism, and it was shown that cTBP could bind stronger to a TTTT box sequence than to the canonical TATA box, especially at high salt concentration. Expand
Bidirectional electron transfer in photosystem I: accumulation of A0- in A-side or B-side mutants of the axial ligand to chlorophyll A0.
TLDR
Ultrafast transient absorbance measurements show that the (A(0)(-) - A(0)) difference signal accumulates in the MH(A684) and MH(B664) mutants under neutral conditions, demonstrating that electron transfer between A( 0)(-) and A(1) is blocked or significantly slowed. Expand
Replacement of the methionine axial ligand to the primary electron acceptor A0 slows the A0- reoxidation dynamics in photosystem I.
TLDR
It is shown that the replacement of either methionine with histidine in the PSI of the unicellular green alga Chlamydomonas reinhardtii resulted in accumulation of A(0)(-) (in 300-ps time scale), suggesting that both the PsaA and PsaB branches are active, and support the model of equivalent electron transfer along both cofactor branches in Photosystem I. Expand
Specific mutation near the primary donor in photosystem I from Chlamydomonas reinhardtii alters the trapping time and spectroscopic properties of P700.
TLDR
The lifetime of the trapping process measured independently by transient absorption and fluorescence spectroscopy in the mutant PSI core antenna is increased by a factor of approximately 2, implying that the trappingprocess in the PSI antenna is limited by the process where the primary donor molecule directly participates. Expand
Site-directed mutations affecting the spectroscopic characteristics and midpoint potential of the primary donor in photosystem I.
TLDR
Estimation of site-directed mutants of the photosystem I reaction center protein PsaB and an amino acid, His-656, that interacts closely with one of the P700 chlorophylls is identified that increases the oxidation midpoint potential of P700/P700+. Expand
Site-directed mutagenesis of the photosystem I reaction center in chloroplasts. The proline-cysteine motif.
TLDR
These procedures have been used to specifically mutate a highly conserved proline-cysteine motif suggested to be important in coordinating the [4Fe-4S] iron-sulfur center Fx, showing that the cysteine is essential for assembly of the photosystem I reaction center although the adjacent proline fulfills no identifiable function. Expand
Site-directed mutagenesis of conserved histidines in the helix VIII domain of PsaB impairs assembly of the photosystem I reaction center without altering spectroscopic characteristics of P700.
TLDR
Protein pulse-labeling shows that changing the histidine residues impairs a post-translational step in reaction center assembly, and the implications of these results to current models of the photosystem I reaction center and related bacterial reaction centers are discussed. Expand
...
1
2
3
4
...