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AAA-ATPase p97/Cdc48p, a Cytosolic Chaperone Required for Endoplasmic Reticulum-Associated Protein Degradation
It is proposed that the role of p97/Cdc48p in ERAD is to act at the cytosolic face of the endoplasmic reticulum and to chaperone dislocation of ERAD substrates and present them to the proteasome. Expand
A Proteasomal ATPase Contributes to Dislocation of Endoplasmic Reticulum-associated Degradation (ERAD) Substrates*
Characterization of Rpt4 involvement in ERAD reveals that it participates in CPY*-HA dislocation, a function previously attributed to p97/Cdc48, another AAA-ATPase essential for ERAD of CPY-HA but dispensable for proteasomal degradation of ΔssCPY-GFP. Expand
Immunological evidence for eight spans in the membrane domain of 3- hydroxy-3-methylglutaryl coenzyme A reductase: implications for enzyme degradation in the endoplasmic reticulum
It is demonstrated that the membrane domain of HMG-CoA reductase spans the ER eight times and are inconsistent with the seven membrane spans topological model, which was proposed to span the ER membrane seven times. Expand
Distinct Steps in Dislocation of Luminal Endoplasmic Reticulum-associated Degradation Substrates
A model that assigns the cytosolic face of the ER as a midpoint to which luminal ERAD substrates emerge and p97/Cdc48p and the proteasome are recruited is proposed, suggesting a role for this chaperone in ERAD. Expand
Synthesis and insertion of cytochrome P-450 into endoplasmic reticulum membranes.
The amino-terminal amino acid sequence of cytochrome P-450 synthesized in an mRNA-dependent system resembles in hydrophobicity the signal segment of presecretory proteins and therefore may serve to insert the polypeptide into the membrane during synthesis. Expand
Proteasomal AAA-ATPases: structure and function.
Light is shed on the assembly of the hetero-hexameric Rpt ring and its consequent spatial arrangement, on the role of Rpt C-termini in opening the 20S 'gate', and on the contribution of each individual Rpt subunit to various cellular processes. Expand
Guidelines and recommendations on yeast cell death nomenclature
Unified criteria for the definition of accidental, regulated, and programmed forms of cell death in yeast based on a series of morphological and biochemical criteria are proposed. Expand
The role of p97/Cdc48p in endoplasmic reticulum-associated degradation: from the immune system to yeast.
  • S. Bar-Nun
  • Biology, Medicine
  • Current topics in microbiology and immunology
  • 2005
P97/Cdc48p, a cytosolic AAA-ATPase that is essential for ERAD, appears to provide the driving force for this process and its ability to physically associate with ERAD substrates, with VIMP and with the E3 gp78 suggest that the p97/ cdc48Ufdl/Npl4 complex acts as a coordinator that maintains coupling between the different steps in ERAD. Expand
Ssz1 Restores Endoplasmic Reticulum-Associated Protein Degradation in Cells Expressing Defective Cdc48–Ufd1–Npl4 Complex by Upregulating Cdc48
The finding that neither pSSZ1 nor pcdc48-10 restored ERAD-L of CPY*-HA, support the conclusion that Ssz1p suppressing effects is brought about by upregulating Cdc48p. Expand
The regulated degradation of 3-hydroxy-3-methylglutaryl-CoA reductase requires a short-lived protein and occurs in the endoplasmic reticulum.
It is demonstrated that inhibition of protein synthesis with cycloheximide abolishes the mevalonate-dependent accelerated degradation of HMGal, in agreement with previous studies which have presented indirect evidence that a short-lived protein is essential for mediating the loss of H MG-CoA reductase activity. Expand