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Cyanuric acid hydrolase: evolutionary innovation by structural concatenation
TLDR
The first X‐ray structure for the cyanuric acid hydrolase, AtzD, is reported, which is a novel fold that likely evolved via the concatenation of monomers of the trimeric YjgF superfamily and the acquisition of a metal binding site.
The structure of the hexameric atrazine chlorohydrolase AtzA
The structure of atrazine chlorohydrolase (AtzA) is presented and is used to reinterpret data from genetic, biochemical and evolutionary studies, providing insight into why this recently evolved
Oxidative Catabolism of (+)-Pinoresinol Is Initiated by an Unusual Flavocytochrome Encoded by Translationally Coupled Genes within a Cluster of (+)-Pinoresinol-Coinduced Genes in Pseudomonas sp.
TLDR
Comp comparative genomics, proteomics, protein semipurification, and heterologous expression are used to identify a flavoprotein from the vanillyl alcohol oxidase/p-cresol methyl hydroxylase (VAO/PCMH) enzyme family in SG-MS2 that carries out the initial hydroxymation of (+)-pinoresinol at the benzylic carbon.
X-Ray Structure of the Amidase Domain of AtzF, the Allophanate Hydrolase from the Cyanuric Acid-Mineralizing Multienzyme Complex
TLDR
Interestingly, it is shown that AtzF forms a large, ca.
Cloning of a Novel 6-Chloronicotinic Acid Chlorohydrolase from the Newly Isolated 6-Chloronicotinic Acid Mineralizing Bradyrhizobiaceae Strain SG-6C
TLDR
Evidence is provided that 6-chloronicotinic acid mineralizing bacterium isolated from enrichment cultures originating from imidacloprid-contaminated soil samples was mobilized into this bacterium by an Integrative and Conjugative Element that feeds 6-hydroxynicotinic Acid into the existing nicotinic acids mineralization pathway.
Binary combinations of organophosphorus and synthetic pyrethroids are more potent acetylcholinesterase inhibitors than organophosphorus and carbamate mixtures: An in vitro assessment.
Anticholinesterase insecticides such as organophosphorous (OP) and carbamates pesticides (CB); and synthetic pyrethroids (SP) pesticides commonly co-occur in the environment. This raises the
Isolation of the (+)-Pinoresinol-Mineralizing Pseudomonas sp. Strain SG-MS2 and Elucidation of Its Catabolic Pathway
TLDR
Evidence suggests that both routes of lactone metabolism lead to vanillin and vanillic acid, which it is shown can then be mineralized by strain SG-MS2, and the oxidative catabolism of (+)-pinoresinol is fundamentally different from the reductive cometabolism reported for two previously characterized bacteria.
High-Resolution X-Ray Structures of Two Functionally Distinct Members of the Cyclic Amide Hydrolase Family of Toblerone Fold Enzymes
TLDR
It is confirmed that members of the CyAH family have the unusual Toblerone fold, albeit with some significant differences, and two new structures are presented: a barbituric acid hydrolase and an enzyme of unknown function.
X-Ray Structure and Mutagenesis Studies of the N-Isopropylammelide Isopropylaminohydrolase, AtzC
TLDR
A plausible catalytic mechanism is proposed, consistent with the biochemical and crystallographic data obtained that is similar to that found in carbonic anhydrase and other members of subtype III of the amidohydrolase family.
Crystallization and preliminary X-ray diffraction analysis of the amidase domain of allophanate hydrolase from Pseudomonas sp. strain ADP
The amidase domain of the allophanate hydrolase AtzF from Pseudomonas sp. strain ADP has been crystallized and preliminary X-ray diffraction data have been collected.
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