Accurate Whole Human Genome Sequencing using Reversible Terminator Chemistry
- D. Bentley, S. Balasubramanian, Anthony J. Smith
- BiologyNature
- 27 October 2008
An approach that generates several billion bases of accurate nucleotide sequence per experiment at low cost is reported, effective for accurate, rapid and economical whole-genome re-sequencing and many other biomedical applications.
Prevalence of quadruplexes in the human genome
- J. Huppert, S. Balasubramanian
- BiologyNucleic Acids Research
- 24 May 2005
There is a significant repression of quadruplexes in the coding strand of exonic regions, which suggests that quadruplex-forming patterns are disfavoured in sequences that will form RNA.
Quantitative visualization of DNA G-quadruplex structures in human cells.
- Giulia Biffi, D. Tannahill, J. McCafferty, S. Balasubramanian
- Biology, ChemistryNature Chemistry
- 1 March 2013
It is shown explicitly that G-quadruplex formation in DNA is modulated during cell-cycle progression and that endogenous G- quadruplex DNA structures can be stabilized by a small-molecule ligand and corroborate the application of stabilizing ligands in a cellular context to target G- Quadruplexes and intervene with their function.
High-throughput sequencing of DNA G-quadruplex structures in the human genome
- V. S. Chambers, Giovanni Marsico, J. M. Boutell, M. Di Antonio, G. Smith, S. Balasubramanian
- BiologyNature Biotechnology
- 20 July 2015
A high-resolution sequencing–based method is presented to detect G4s in the human genome and observed a high G4 density in functional regions, as well as in genes previously not predicted to contain these structures (such as BRCA2).
Targeting G-quadruplexes in gene promoters: a novel anticancer strategy?
- S. Balasubramanian, L. Hurley, S. Neidle
- Biology, ChemistryNature reviews. Drug discovery
- 1 April 2011
The evidence for G-quadruplexes in gene promoters is described and their potential as therapeutic targets are discussed, as well as progress in the development of strategies to harness this potential through intervention with small-molecule ligands.
G-quadruplex structures mark human regulatory chromatin
- Robert Hänsel-Hertsch, D. Beraldi, S. Balasubramanian
- BiologyNature Genetics
- 12 September 2016
The development of G4 ChIP–seq, an antibody-based G4 chromatin immunoprecipitation and high-throughput sequencing approach, shows that regulatory, nucleosome-depleted chromatin and elevated transcription shape the endogenous human G4 DNA landscape.
G-quadruplexes in promoters throughout the human genome
- J. Huppert, S. Balasubramanian
- BiologyNucleic Acids Research
- 14 December 2006
It is shown that the promoter regions (1 kb upstream of the transcription start site TSS) of genes are significantly enriched in quadruplex motifs relative to the rest of the genome, with >40% of human gene promoters containing one or more quadruplexaterials.
Small molecule-induced DNA damage identifies alternative DNA structures in human genes
- R. Rodriguez, K. Miller, S. Jackson
- BiologyNature Chemical Biology
- 5 February 2012
It is shown that the G-quadruplex interacting drug pyridostatin promoted growth arrest in human cancer cells via inducing replication- and transcription-dependent DNA damage.
An RNA G-quadruplex in the 5' UTR of the NRAS proto-oncogene modulates translation.
- Sunita Kumari, A. Bugaut, J. Huppert, S. Balasubramanian
- Biology, ChemistryNature Chemical Biology
- 1 April 2007
A highly conserved, thermodynamically stable RNA G-quadruplex is discovered in the 5' untranslated region (UTR) of the gene transcript of the human NRAS proto-oncogene and it is demonstrated that this NRAS RNAG- quadruplex modulates translation.
Quantitative Sequencing of 5-Methylcytosine and 5-Hydroxymethylcytosine at Single-Base Resolution
- M. Booth, M. Branco, S. Balasubramanian
- Biology, ChemistryScience
- 18 May 2012
Distinguishing Epigenetic Marks Methylation of the cytosine base in eukaryotic DNA (5mC) is an important epigenetic mark involved in gene silencing and genome stability. Methylated cytosine can be…
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