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Cloning, structure, and expression of the mitochondrial cytochrome P-450 sterol 26-hydroxylase, a bile acid biosynthetic enzyme.
TLDR
The structure of the sterol 26-hydroxylase cDNA reveals it to be a mitochondrial cytochrome P-450, and blotting experiments revealed that the mRNA for this enzyme is expressed in many tissues and that it is encoded by a low copy number gene in the rabbit genome. Expand
Male pseudohermaphroditism caused by mutations of testicular 17β–hydroxysteroid dehydrogenase 3
TLDR
Four substitution and two splice junction mutations were identified in the 17βHSD3 genes of five unrelated male pseudohermaphrodites that severely compromised the activity of the 17 β–HSD type 3 isozyme. Expand
Biochemical Properties of Purified Recombinant Human β-Carotene 15,15′-Monooxygenase*
TLDR
The current data suggest that the human BCO enzyme may, in addition to its well established role in the digestive system, also play a role in peripheral vitamin A synthesis from plasma-borne provitamin A carotenoids. Expand
Structural and biochemical properties of cloned and expressed human and rat steroid 5 alpha-reductases.
TLDR
Both enzymes expressed in COS cells showed similar substrate specificities for naturally occurring steroid hormones, however, synthetic 4-azasteroids demonstrated marked differences in their abilities to inhibit the human and rat steroid 5 alpha-reductases. Expand
Deletion of steroid 5α-reductase 2 gene in male pseudohermaphroditism
TLDR
The results verify the existence of at least two 5α-reductases in man and provide insight into a fundamental hormone-mediated event in male sexual differentiation. Expand
Neuronal expression and subcellular localization of cholesterol 24‐hydroxylase in the mouse brain
TLDR
Findings reveal the microsomal localization of 24‐hydroxylase and provide subcellular insight into cholesterol turnover in the brain and reveal impaired learning and defective hippocampal long‐term potentiation in mice deficient in this enzyme. Expand
Expression cloning and regulation of steroid 5 alpha-reductase, an enzyme essential for male sexual differentiation.
TLDR
DNA sequence analysis demonstrates that the liver and ventral prostate forms of steroid 5 alpha-reductases are identical hydrophobic proteins of 29 kDa, and suggests that the steroid 5AlphaReductase gene is differentially regulated by testosterone in androgen-responsive versus non-responsive tissues. Expand
17 beta-Hydroxysteroid dehydrogenase type 2: chromosomal assignment and progestin regulation of gene expression in human endometrium.
TLDR
The chromosomal localization of human 17 beta-HSD type 2, the expression of this gene in human endometrium, and the tissue distribution of the mRNA are determined, consistent with the view that the progestin-regulated 17 alpha-hydroxyl group of the glandular epithelium of the human Endometrium is primarily, if not exclusively, the product of the 17beta-HSd type 2 gene. Expand
Deficient 17β-Hydroxysteroid Dehydrogenase Type 2 Expression in Endometriosis: Failure to Metabolize 17β-Estradiol
TLDR
It follows that the product of the aromatase reaction, namely estrone, that is weakly estrogenic can be converted to the potent estrogen, 17β-estradiol, in endometriotic tissues. Expand
Inhibition of rat α-reductases by finasteride: Evidence for isozyme differences in the mechanism of inhibition
TLDR
The mechanism of inhibition of the rat types 1 and 2 5alpha-reductase by finasteride was investigated using recombinantly expressed enzymes and whole cell assays confirm differences in mechanism. Expand
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