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Essential functions and actin‐binding surfaces of yeast cofilin revealed by systematic mutagenesis
Biochemical characterization of interactions between nine mutant yeast cofilins and yeast actin provided evidence that F‐actin binding and depolymerization are essential cofilin functions, and mutation of the cofilmin N‐terminus suggests that its sequence is conserved because of its critical role in actin interactions.
Type VI secretion apparatus and phage tail-associated protein complexes share a common evolutionary origin
It is proposed that T6SS is a multicomponent structure whose extracellular part resembles both structurally and functionally a bacteriophage tail, an efficient machine that translocates proteins and DNA across lipid membranes into cells.
VISTA, a novel mouse Ig superfamily ligand that negatively regulates T cell responses
VISTA suppresses T cell proliferation and cytokine production and can influence autoimmunity and antitumor responses in mice.
Structural basis for co-stimulation by the human CTLA-4/B7-2 complex
Direct observation of this CTLA-4/B7-2 network provides a model for the periodic organization of these molecules within the immunological synapse and suggests a distinct mechanism for signalling by dimeric cell-surface receptors.
Protein production and purification
This review presents methods that could be applied at the outset of any project, a prioritized list of alternate strategies and a list of pitfalls that trip many new investigators.
A high-affinity interaction with ADP-actin monomers underlies the mechanism and in vivo function of Srv2/cyclase-associated protein.
It is suggested that in vivo CAP competes with cofilin for binding ADP-actin monomers, allows rapid nucleotide exchange to occur on actin, and then allows other cellular factors such as profilin to take the handoff of ATP- actin and facilitate barbed end assembly.
Identification of a second aryl phosphate-binding site in protein-tyrosine phosphatase 1B: a paradigm for inhibitor design.
Identifying a second pTyr molecule at the same site in the PTP1B/C215S-pTyr complex confirms that these residues constitute a low-affinity noncatalytic aryl phosphate-binding site, which provides a paradigm for the design of tight-binding, highly specific P TP1B inhibitors that can span both the active site and the adjacent noncatalyst site.
ZBP1 recognition of beta-actin zipcode induces RNA looping.
The RNA looping induced by ZBP1 binding provides a mechanism for specific recognition and may facilitate the assembly of post-transcriptional regulatory complexes by remodeling the bound transcript.
The structure of nonvertebrate actin: Implications for the ATP hydrolytic mechanism
This work aims to demonstrate the efforts towards in-situ applicability of x-ray diffraction-spot assisted, 3D image analysis to provide real-time information about the response of the immune system to x-rays.