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Cypress (Cupressus sempervirens) pollen allergens: identification by protein blotting and improved detection of specific IgE antibodies.
On the basis of results of an investigation of the effects of different treatments employed, a dialysed and reduced extract of Cupressus sempervirens was separated electrophoretically on sodiumExpand
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Comparison of different blocking agents and nitrocelluloses in the solid phase detection of proteins by labelled antisera and protein A.
Five different brands of nitrocellulose (NC), each of pore size 0.45 micron and without adsorbed antigen, bound different amounts of two labelled antisera and labelled protein A. Experiments withExpand
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Identification of Parietaria judaica pollen allergens.
Parietaria judaica pollen allergens, fractionated by SDS-polyacrylamide gel electrophoresis and transferred to nitrocellulose membranes, were identified using 52 sera collected in Australia andExpand
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A re-examination of ryegrass (Lolium perenne) pollen allergens.
Electroblotting of crude ryegrass pollen extracts and purified group I, II and III allergens identified 14 IgE-binding components, 8 of which were previously unrecognized. In addition to allergenExpand
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Identification of orchard grass (Dactylis glomerata) pollen allergens following electrophoretic transfer to nitrocellulose.
Orchard grass (cocksfoot) pollen extracts, fractionated by polyacrylamide gradient electrophoresis or SDS gel electrophoresis were electroblotted onto nitrocellulose membranes and probed with seraExpand
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Identification of Bermuda grass (Cynodon dactylon)--pollen allergens by electroblotting.
  • S. A. Ford, B. Baldo
  • Chemistry, Medicine
  • The Journal of allergy and clinical immunology
  • 1 May 1987
Bermuda grass-pollen proteins were electrophoretically separated on polyacrylamide gels and transferred to nitrocellulose where IgE-binding components were detected by reaction with individualExpand
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Protein blotting on nitrocellulose: some important aspects of the resolution and detection of antigens in complex extracts.
The resolution and detection of individual components in complex extracts by protein blotting have been investigated. By probing nitrocellulose transfers with monospecific and multispecific antisera,Expand
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Identification of jack‐jumper ant (Myrmecia pilosula) venom allergens
Jack‐jumper ant venom proteins were etectrophoretically separated on SDS‐polyacry‐lamide gels, transferred to nitrocellulose and probed with sera from subjects who had experienced an allergicExpand
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Enhanced immunodetection of blotted house dust mite protein allergens on nitrocellulose following blocking with tween 20
The effect of blocking nitrocellulose membranes with the nonionic detergent Tween 20 on the detection, by protein blotting, of IgE‐binding to house dust mite Dermatophagoides pteronyssinus allergensExpand
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The action of (S)-alpha-chlorohydrin and 6-chloro-6-deoxyglucose on the metabolism of guinea pig spermatozoa.
(S)-alpha-Chlorohydrin inhibits the conversion of fructose to lactate by mature guinea pig spermatozoa in vitro. At a concentration of 2mM, there is a specific inhibition of glyceraldehyde-phosphateExpand
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