S. Yu. Kuznetsova

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159 As early as in 1953, nucleic acids were reported to produce water-insoluble salts with Cetavlon (Cetavlon is the commercial name of hexadecyltrimethylammonium bromide) [1]. This property of nucleic acids is the basis for the widespread Cetavlon method for isolating DNA from biological sources (e.g., see [2, 3]). This method takes advantage of the fact(More)
A generic method is described for the fluorescence "readout" of the activity of single redox enzyme molecules based on Förster resonance energy transfer from a fluorescent label to the enzyme cofactor. The method is applied to the study of copper-containing nitrite reductase from Alcaligenes faecalis S-6 immobilized on a glass surface. The parameters(More)
A method for fluorescence detection of a protein's redox state based on resonance energy transfer from an attached fluorescence label to the prosthetic group of the redox protein is described and tested for proteins containing three types of prosthetic groups: a type-1 copper site (azurin, amicyanin, plastocyanin, and pseudoazurin), a heme group (cytochrome(More)
Generation of functionally active biomolecular monolayers is important in both analytical science and biophysical analyses. Our ability to monitor the redox-active state of immobilized proteins or enzymes at a molecular level, from which stochastic and surface-induced variations would be apparent, is impeded by comparatively slow electron-transfer kinetics(More)
Sulfated glycosaminoglycsns are known to stimulate regeneration of the skin when injured [3, 5]. This property also is shared by preparations for injection (rumalon, mucartrin, etc.) and by substances for external application (chonsuride) [2]. In the Soviet Union only one preparation based on chondroitin sulfate (ChS) is marketed, namely chonsuride, which(More)
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