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The role of the cytoskeletal structure on the shear tolerance of hybridoma and insect cells to laminar and turbulent shear flow field was investigated. The cytoskeletal network of cells modified by taxol and diamide, which are known to alter the stability of specific cytoskeletal components, resulted in increased cell shear tolerance to both laminar and(More)
T-vectors play an important role in cloning of polymerase chain reaction products. In the present study, a novel pUEG-T vector was developed using enhanced green fluorescent protein (EGFP) as an indicator. To improve EGFP-based green-white screening, the lipoprotein mutant promoter, a strong constitutive promoter, was utilized to control the expression of(More)
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