S W Hillard

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The newly developed flow instrument, bypass trapped-flow analysis system (ByT-FAS), was used to perform enzyme assays and generate data for evaluation of an enzyme's kinetic parameters. The enzyme assay was yeast hexokinase coupled to glucose-6-phosphate dehydrogenase and the production of reduced NADPH was monitored fluorometrically. The kinetic parameters(More)
Bypass trapped flow analysis system (ByT-FAS) is a new trapped flow apparatus designed to perform chemical measurements with flow injection systems at physical steady state using small volumes (10-150 mul) of injected sample and reagent. We have used a micro-volume version of ByT-FAS instrumentation with a chemiluminescent detection system to quantitate the(More)
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