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Depolarization of neuron membrane was shown to occur during cAMP injection by means of both iontophoresis and pressure. Distension of the neuron by means of blowing with large volumes of solution without cAMP can produce reversible responses. The time course can be made similar to that of the cAMP effect and the responses can be repeated many times in the(More)
The depolarization of neuronal membrane during cAMP injection by means of both iontophoresis and pressure was shown. Neuron blowing by solutions without cAMP gave the similar effect. The effect of cAMP can be differentiated in the presence of phosphodiesterase inhibitors. The experiments support the hypothesis that cyclic nucleotides and mechanical(More)
Cyclic 3',5'-AMP and cyclic 3',5'-GMP injected into large neurons of the snail Helix lucorum altered neuron activity. The effect of cAMP is usually depolarizing and that of cGMP hyperpolarizing. The results are specific for 3',5'-cyclic nucleotides. The experiments support the hypothesis that reaction-diffusion processes involving cyclic nucleotides form(More)
Investigation of the influence of cAMP on neuronal electric activity suggests that nerve cells can solve problems using an intraneuronal calculating medium based on the cytoskeleton. When a new problem is posed, this structure has to be disassembled and assembled by the neuronal molecular computer according to the program recorded in DNA. If DNA lacks an(More)
Intracellular microiontoforetic injections of cyclic 3',5'-AMP and cyclic 3',5'-GMP elicit different responses in the same neuron. Drugs were injected by current of 1--10 nA using 3-barrel micropipettes. Unlike cyclic 3',5'-AMP arising the depolarization of the neuron, cyclic 3',5'-GMP usually hyperpolarizes it. An inhibitor of phosphodiesterases,(More)
Intracellular cAMP injection and negative pressure in the patch-electrode increase the interburst closed time of the same potassium ionic channels in the snail neuron membrane. Sodium channels which were registered as change of background noise are activated both by cAMP injection and by negative pressure. These results are considered in connection with(More)
Drugs preventing cAMP interaction with regulatory subunit of cAMP-dependent protein kinase, tolbutamide and db-cAMP injected into neurons of Helix lucorum decreased the cell response to cAMP, but H-8-a potent inhibitor of this enzyme catalytic subunit did not produce such effect. It is suggested that the neuron electric response to cAMP injection is not(More)
Influence of different metabolic poisons on cAMP-evoked neuron membrane permeability is investigated. Drugs preventing cAMP binding with R subunits of protein kinase decrease the cAMP-evoked current, but the inhibitor of the C subunit. H8, has no effect. The cAMP-dependent current is increased by uncouplers and decreased by inhibitors of glycolysis and(More)
Injection of cAMP induces in snail neurons generator potential, which is related to an increase of sodium and decrease of potassium permeability of the neuron outer membrane. A model is proposed which takes into account cAMP diffusion inside the neuron from the injection place and interaction of these molecules with the intercellular system controlling(More)