S. Schuler

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Metabolomics and systems biology require the acquisition of reproducible, robust, reliable, and homogeneous biological data sets. Therefore, we developed and validated standard operating procedures (SOPs) for quenching and efficient extraction of metabolites from Escherichia coli to determine the best methods to approach global analysis of the metabolome.(More)
Fourier transform infrared (FT-IR) spectroscopy was employed as a rapid high-throughput phenotypic typing technique to generate metabolic fingerprints of Escherichia coli MG1655 pDTG601A growing in fed-batch culture, during the dioxygenase-catalysed biotransformation of toluene to toluene cis-glycol. With toluene fed as a vapour, the final toluene(More)
Whole cells are usually used for oxygenase-catalysed biotransformations to ensure efficient cofactor recycling and to avoid problems with purification and stability of these complex, multi-subunit enzymes. Productivity in oxygenase-catalysed biotransformations is frequently restricted by toxicity of the substrates to the cells, but attempting to solve this(More)
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