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In eukaryotes, the Cdt1-bound replicative helicase core MCM2-7 is loaded onto DNA by the ORC-Cdc6 ATPase to form a prereplicative complex (pre-RC) with an MCM2-7 double hexamer encircling DNA. Using purified components in the presence of ATP-γS, we have captured in vitro an intermediate in pre-RC assembly that contains a complex between the ORC-Cdc6 and(More)
Mobile personal communications applications require high speed, light weight, low power and customis-ability. They also exhibit architectural heterogeneity of subsystems. This paper describes a design methodology for such applications which spans the whole design process; from specification, through simulation to synthesis. The objectives of this(More)
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