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An enzyme-linked immunosorbent assay (ELISA) with high sensitivity was developed for use in the avian system. Anti-reovirus antibody produced in chickens against the isolants S1133, UMI-203, Reo-25, and Winterfield were detected with this method at high titers (up to 1:14,000).
A microplate enzyme-linked immunosorbent assay was developed to detect chicken anti-rovirus antibodies. Studies of the parameters which affect the outcome of the assay with avian serum revealed two aspects for a successful assay. First, enzyme-antibody conjugates prepared by the periodate oxidation technique were found to have retained far more(More)
The need to design diagnostic systems in parallel with prime systems has become commonplace. During one such program, an approach to defining and sharing needed information that is both simple and effective was found. Accomplishment in parallel of operational system and automated test equipment (ATE) designs in a concurrent engineering environment requires(More)
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