S S Goldenberg

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Nonneuronal cells complicate the study of neurons in vitro. A pure population of viable neurons can be obtained easily using gradients of Percoll. For each experiment, 20 dorsal root ganglia (DRG) are minced, then sequentially dissociated in collagenase and trypsin, which digest all the intercellular connections. The dissociated tissue is separated first on(More)
Carbonic anhydrase (CAH) was localized in the mitochondria-rich cells (MRC) of 1-week-old salamander larvae gill epithelium, in both MRC and pavement cells of 6-week-old larvae, and in regenerated stems of previously amputated gills. CAH activity of the MRC was measured quantitatively using a microscope densitometric technique. Changes in CAH activity per(More)
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