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A stopped-flow investigation of the electron-transfer reaction between oxidized azurin and reduced Pseudomonas aeruginosa cytochrome c-551 oxidase and between reduced azurin and oxidized Ps. aeruginosa cytochrome c-551 oxidase was performed. Electrons leave and enter the oxidase molecule via its haem c component, with the oxidation and reduction of the haem(More)
Some spectra of Pseudomonas cytochrome oxidase are reported, both for comparison with those of other workers and to illustrate the differences between the ascorbate- and dithionite-reduced forms of the enzyme. A spectrum of the reduced enzyme-CO complex, prepared in the absence of added reductants by incubation under CO, is also included.(More)
The binding of cyanide to both oxidized and ascorbate-reduced forms of Pseudomonas cytochrome c-551 oxidase was investigated. Spectral studies on the oxidized enzyme and its apoprotein showed that the ligand can bind to both the c and d, haem components of the molecule, and kinetic observations indicated that both chromophores reacted, under a variety of(More)
The production of the soluble cytochrome oxidase/nitrite reductase in the bacterium Pseudomonas aeruginosa is favoured by anaerobic conditions and the presence of KNO3(20g/l) in the culture medium. Of three methods commonly used for the disruption of bacterial suspensions (ultrasonication, liquid-shear homogenization and glass-bead grinding), sonication(More)
The binding of CO to ascorbate-reduced Pseudomonas cytochrome oxidase was investigated by static-titration, stopped-flow and flash-photolytic techniques. Static-titration data indicated that the binding process was non-stoicheiometric, with a Hill number of 1.44. Stopped-flow kinetics obtained on the binding of CO to reduced Pseudomonas cytochrome oxidase(More)
The goal of the High-Level Architecture (HLA) is to promote interoperability and reuse between federates. In this context, HLA fills the role of middleware in the domain of distributed simulation. However, in many respects, HLA falls short of its promised potential. These problems can be attributed in part to the HLA standard itself, which leaves a number(More)
This paper presents the results of an investigation of employing configurable scalar and vector coprocessors to accelerate the G.723.1 and the G.729A speech coders. Architecture exploration has produced a reduction by up to 70% of the total number of instructions executed following the introduction of custom instructions. The accelerators are designed to be(More)
This work quantifies the performance benefit of vectorized versions of the ITU-T G.729A and G.723.1 speech coding standards. Architecture-level experimentation with the addition of custom vector instructions indicates a reduction in the dynamic instruction count of the workloads of the order of 51% and 65% respectively at a vector register length of sixteen(More)