S. Paglini

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A fraction from Swiss albino mouse brain homogenate obtained by DEAE cellulose chromatography showed a non-specific hemagglutination-inhibitory property for some Togaviruses. Evidence are presented indicating that the inhibitory property is related to a protein-rich fraction with sialic acid as an active group.
The brain gangliosides mixture of Swiss albino mice were studied from a biophysical viewpoint. The value of v found was 0.577 ml/g. The isoelectric point gave a value of 1.50, which is in agreement with the presence of sialic acid groups. The isoionic point, 2.76, is close to the pKa for sialic acid. The number average molecular weight determined in the(More)
Gangliosides isolated from the brain of ten-day-old Swiss albino mice were purified by thin layer chromatography, and the resulting purified gangliosides were tested for their ability to inhibit hemagglutination by Eastern Equine Encephalitis virus. The trisialoganglioside and the more rapidly migrating of the two disialogangliosides were found to inhibit(More)
In this study, we describe the isolation and partial characterization of a Pixuna virus receptor, which is a component of a plasma membrane fraction of chicken embryo fibroblast (CEF). Polyclonal antiserum was prepared from rabbits immunized with the membrane fraction. Said polyclonal antiserum reacted in a similar way as monoclonal antibodies raised(More)
We studied 20 strains of Pseudomonas aeruginosa in its ability to produce proteolytic enzymes. Three different culture media were used: one containing salts and yeast; another one, the dialysed brain-heart-broth fluid and the third, brain-heart-broth. The best was the last one. The purification of the enzymes was performed by precipitation with ammonium(More)