Montserrat Elías-Arnanz8
María Angeles Jiménez3
Juan Manuel Ortiz-Guerrero3
Aránzazu Gallego-García2
8Montserrat Elías-Arnanz
3María Angeles Jiménez
3Juan Manuel Ortiz-Guerrero
2Aránzazu Gallego-García
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Direct targeting of critical DNA-binding elements of a repressor by its cognate antirepressor is an effective means to sequester the repressor and remove a transcription initiation block. Structural descriptions for this, though often proposed for bacterial and phage repressor-antirepressor systems, are unavailable. Here, we describe the structural and(More)
CarD, a global transcriptional regulator in Myxococcus xanthus, interacts with CarG via CarDNter, its N-terminal domain, and with DNA via a eukaryotic HMGA-type C-terminal domain. Genomic analysis reveals a large number of standalone proteins resembling CarDNter. These constitute, together with the RNA polymerase (RNAP) interacting domain, RID, of(More)
  • Marco Jost, Jésus Fernández-Zapata, María Carmen Polanco, Juan Manuel Ortiz-Guerrero, Percival Yang-Ting Chen, Gyunghoon Kang +3 others
  • 2015
Photoreceptor proteins enable organisms to sense and respond to light. The newly discovered CarH-type photoreceptors use a vitamin B12 derivative, adenosylcobalamin, as the light-sensing chromophore to mediate light-dependent gene regulation. Here we present crystal structures of Thermus thermophilus CarH in all three relevant states: in the dark, both free(More)
Thermus thermophilus transcriptional factor TtCarH belongs to a newly discovered class of photoreceptors that use 5′-deoxyadenosylcobalamin (AdoB12) as the light-sensing chromophore. Photoregulation relies on the repressor activity of AdoB12-bound oligomers in the dark, which light counteracts by oligomer disruption due to AdoB12 photolysis. In this study,(More)
  • Diego Bernal-Bernal, Aránzazu Gallego-García, Gema García-Martínez, Francisco García-Heras, María Angeles Jiménez, S. Padmanabhan +1 other
  • 2015
Two prototypes of the large CarD_CdnL_TRCF family of bacterial RNA polymerase (RNAP)-binding proteins, Myxococcus xanthus CarD and CdnL, have distinct functions whose molecular basis remain elusive. CarD, a global regulator linked to the action of several extracytoplasmic function (ECF) σ-factors, binds to the RNAP β subunit (RNAP-β) and to protein CarG via(More)
  • Aránzazu Gallego-García, Yasmina Mirassou, Diana García-Moreno, Montserrat Elías-Arnanz, María Angeles Jiménez, S. Padmanabhan +1 other
  • 2014
CdnL and CarD are two functionally distinct members of the CarD_CdnL_TRCF family of bacterial RNA polymerase (RNAP)-interacting proteins, which co-exist in Myxococcus xanthus. While CarD, found exclusively in myxobacteria, has been implicated in the activity of various extracytoplasmic function (ECF) σ-factors, the function and mode of action of the(More)
The coenzyme B12-dependent photoreceptor protein, CarH, is a bacterial transcriptional regulator that controls the biosynthesis of carotenoids in response to light. On binding of coenzyme B12 the monomeric apoprotein forms tetramers in the dark, which bind operator DNA thus blocking transcription. Under illumination the CarH tetramer dissociates, weakening(More)
—WindSat, the first polarimetric microwave radiometer on orbit, has as its primary objective the demonstration of robust retrieval of the sea surface wind vector from measured brightness temperatures. The ocean surface wind vector is one of the key environmental data records for the NPOESS Conical Microwave Imager/Sounder (CMIS) instruments, first planned(More)
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