S N Wickramasinghe

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AIM To study the value of serum holo-transcobalamin II (holo-TCII) measurements in the differential diagnosis of macrocytosis. METHODS Holo-TCII concentrations were measured in serum samples from 50 healthy non-vegetarian subjects and 30 patients with macrocytosis, using a technique based on the adsorption of holo-TCII with amorphous, precipitated silica.(More)
A non-dialysable cytotoxic activity developed in the supernatants of human blood-monocyte-derived macrophages cultured in the presence of ethanol and in the serum of three healthy volunteers who drank 500-700 ml wine over 20-35 min. On 'Sephacryl S-300' gel filtration of the culture supernatants and the serum samples from the subjects who took alcohol, the(More)
The effects of a number of additives on the rate of conversion of ethanol (1 mg/ml; 21.7 mM) to acetate by monolayers of blood monocyte-derived human macrophages were investigated. The additives studied were superoxide dismutase (SOD; 1,500 U/ml), catalase (1,500 U/ml), tetrahydrofurane (20 mM), and PMA (20 nM), either singly or in various combinations.(More)
An inherited hypochromic microcytic anemia transmitted in an autosomal manner has been observed in three generations of an English family. Affected members had the hallmarks of heterozygous beta-thalassemia, ie, elevated levels of hemoglobin A2 and imbalanced globin chain synthesis. However, despite extensive sequence analysis, no mutations could be found(More)
inhibitor associated with chronic-alpha therapy in a patient with C. Fabbri haemophilia A. Nontransfusional Iron Overload in Thalassemia: Association With Hereditary Hemochromatosis To the Editor: by Southern blot hybridization. Point mutations causing b thalas-semia were detected by sequence analysis of PCR-amplified b globin genes. 10 Hemochromatosis is(More)
Serum obtained from healthy volunteers 6-7 h after consumption of 60-95 g of ethanol contained cytotoxic activity against mouse A9 cells and all of six human cell lines tested. Affinity chromatography of such sera demonstrated that at least some of the cytotoxic molecules consisted of altered albumin. Complexes formed by the reaction of 14C-acetaldehyde(More)
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