S. Madhusudhanan

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Temperature-sensitive (Ts) mutants are a powerful tool with which to study gene function in vivo. Ts mutants are typically generated by random mutagenesis followed by laborious screening procedures. By using the Escherichia coli cytotoxin CcdB as a model system, simple procedures for generating Ts mutants at high frequency through site-directed mutagenesis(More)
Little is known about mechanisms responsible for the temperature-sensitive (ts) phenotype, or of the transferability of ts mutants of a specific gene between organisms. Using a structure-based approach, nine ts mutants of Gal4 were generated in yeast by mutating four DNA binding residues. Two of these nine yeast ts mutants were cloned into P element vectors(More)
Data mining or knowledge discovery in databases in simple words is the non-trivial extraction of implicit, previously unknown and potentially useful information from data. It deals with the discovery of hidden knowledge, unexpected patterns and new rules from large databases. Knowledge discovery in databases is the process of identifying a valid,(More)
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