S. M. de Looze

  • Citations Per Year
Learn More
The selective staining of a single butyrylesterase, following isoelectric focusing of red cell lysates from 14 mammalian species, including man, was achieved using the chromogenic substrate n-acetyl-l-alanine-α-naphthyl ester. This procedure optimized the identification of this enzyme, and a close correspondence of its properties was observed in all the(More)
ES-201 was isolated from male mouse kidney and purified 350-fold by ion-exchange chromatography, isoelectric focusing, and gel filtration. The resultant product was apparently homogeneous by the criteria of polyacrylamide gel electrophoresis and immunodiffusion and represented a major fraction of male mouse kidney esterase. Sodium dodecyl sulfate gel(More)
Esterase-6 in fresh homogenates of heart muscle and testis of the house mouse shows a two band (C allele) or three band (A allele) pattern in disc electrophoresis. These primary bands generated a series of secondary bands upon lowering the pH of the homogenates, and the secondary pattern, possibly resulting from partial proteolysis, was seen in varying(More)
A unique recombination is described between (Es-1, Es-6) and (Es-9, Es-22) within gene cluster 1 of the esterase gene region on chromosome 8 of the house mouse. This recombination established the gene orderEs-1—Es-6—(Es-9, Es-22)—Got-2. A further 73 recombinations, from a total of 911 backcrosses, had taken place between cluster 1 and cluster 2. A distance(More)
Ten different nonspecific esterases in both mouse (Mus musculus) and rat (Rattus norvegicus) testis were identified following the analysis of electrophoretic patterns using genetic, developmental, and biochemical criteria. None of the enzymes were unique to testis, although the pattern of activity was testis specific. The enzymes comprised, in each species,(More)
  • 1