S. M. Bychkov

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The method has been developed for preparing complexes of lysozyme with chondroitin sulfate-4 and -6, non-aggregated (soluble) proteoglycans, cartilage proteoglycan aggregates, heparin fractions containing residues 3(H-3) and 4(H-4) of sulfuric acid per dimer of polymer. The studies of the chemical composition and IK-spectra of proteoglycan-lysozyme(More)
The infrared spectra of hyaluronic acid (HA), protein chondroitin keratan sulfate (PCKS), aggregates of proteoglycans (AP), glucuronic acid and its potassium salt, glucosamine, galactosamine (both as hydrochlorides), N-acetyl-glucosamine, N-acetyl-galactosamine, ammonium salt of N-acetyl-neuraminic acid were obtained in the region 200--4000 cm-1. It was(More)
The action of hyaluronate potassium (HUK) and of protein chondroitin-4-sulphate potassium (PCHSK) on the aggregation and adhesion of rabbit erythrocytes suspended in physiological saline was studied. It was found that the capacity of HUK and PCHSK to produce an unspecific and reversible aggregation of erythrocytes was connected with the formation by these(More)
Much of the protein-chondroitin-keratin-sulfate (PCKS; the aggregated, insoluble fraction) in cartilage is contained in the form of a noncovalent compound composed of many (20i00 or more) macromolecules of this proteoglycan with one macromolecule of hya!uronic acid (HUA). These proteoglycan aggregates (PA) are formed through interaction of the(More)
The infrared spectra of standard hyaluronic acid, proteochondroitin sulfate, aggregates of proteoglycans and spectra of the vitreous body, cornea and sclera were obtained in the region of 4000-400 cm-1. The comparative analysis of the spectra of these tissues and spectra made it possible to identify greater absorption bands of these biopolymers.
The infrared spectra of the hyalin cartilage vitreous body, cornea, sclera and spectra of standard normal Na(+)-salts of hyaluronic acid, chondroitin-4-sulfate, chondroitin-6-sulfate, protein-chondroitin-keratan-sulfate, aggregates of proteoglycans of the hyalin cartilage, heparin fractions containing 3 and 4 residues of sulfuric acid per dimer of polymer(More)
The authors have devised the methods for preparing free hyaluronic acid (HA) and non-aggregating fraction of protein-chondroitin-keratan sulfate (PCKS), as well as those for preparing their Na+, K+, Ca2+ and Mg2+ salts (acid and neutral). Infrared spectroscopy has demonstrated the presence of intermolecular hydrogen bonds, formed by hydroxyl groups, in HA(More)