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We describe here the isolation and biochemical characterization of a population of protein aggregates from the postsynaptic density (PSD) prepared from pig cerebral cortex. The protein constituents of these aggregates are linked together primarily by disulfide bonds. Negative staining electron microscopy revealed that the isolated protein aggregates were(More)
Small cell lung cancer (SCLC) has been associated with loss of heterozygosity at several distinct genetic loci including chromosomes 3p, 13q, and 17p. To determine whether the retinoblastoma gene (Rb) localized at 13q14, might be the target of recessive mutations in lung cancer, eight primary SCLC tumors and 50 cell lines representing all major histologic(More)
Postsynaptic densities (PSDs) isolated by biochemical means consist of a complex mixture of proteins that tightly bond to each other. The purpose of this report is to study whether the numerous interprotein disulfides found in the isolated PSDs contribute to the tight structure of the PSDs and whether these interprotein disulfides exist in vivo. PSDs were(More)
Exposure to elevated temperature provokes a sequence of events (heat shock response) in all living organisms. Through this response, heat shock proteins (HSPs) are induced and protect the cells against subsequent injury. We investigated the effect of heat treatment on bicuculline-induced convulsions, and analyzed a possible role of HSPs. Screw electrodes(More)
The melting behavior of 0.1–10-nm-thick discontinuous indium films formed by evaporation on amorphous silicon nitride is investigated by an ultrasensitive thin-film scanning calorimetry technique. The films consist of ensembles of nanostructures for which the size dependence of the melting temperature and latent heat of fusion are determined. The(More)
This work investigates the thermodynamic properties of small structures of Al using an ultrasensitive thin-film differential scanning calorimeter. Al thin films were deposited onto a Si 3 N 4 surface via thermal evaporation over a range of thicknesses from 6 to 50 Å. The Al films were discontinuous and formed nanometer-sized clusters. Calorimetry(More)
The interaction of the endogenous K-opioid, dynorphin, with N-methyl-D-aspartate (NMDA) receptors was studied in single periaqueductal gray (PAG) cells using the whole cell patch recording technique. We have found that dynorphin A (1-17) rapidly and reversibly potentiates NMDA-activated currents in a subpopulation of PAG cells. The potentiation cannot be(More)
We introduce a scanning calorimeter for use with a single solid or liquid sample with a volume down to a few nanoliters. Its use is demonstrated with the melting of 52 nL of indium, using heating rates from 100 to 1000 K/s. The heat of fusion was measured to within 5% of the bulk value, and the sensitivity of the measurement was Ϯ7 ␮W. The heat of(More)
This paper analyses the electrical response of a quasi-adiabatic, thin-film nanocalorimeter system for the purpose of obtaining values of the heat capacity of ultra-thin samples. The pulse-heating method of heat capacity measurement is based on the observation of the temperature rise during the heating of the sample by a pulse of current. By measuring the(More)
We have developed a new thin-film differential scanning calorimetry technique that has extremely high sensitivity of 0.2 nJ. By combining two calorimeters in a differential measurement configuration, we have measured the heat capacity and melting process of Sn nanostructures formed via thermal evaporation with deposition thickness down to 1 Å. The(More)