S. K. Bhatia

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T. C. Bhalla2
R. K. Bhatia2
P. K. Mehta2
2T. C. Bhalla
2R. K. Bhatia
2P. K. Mehta
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Alcaligenes sp. MTCC 10674 has a bienzy-matic system for the hydrolysis of nitriles. The nitrile hydratase and amidase have been purified simultaneously to homogeneity using a combination of (NH) 4 SO 4 precipitation , ion exchange chromatography and gel permeation chromatography. Nitrile hydratase and amidase have molecular weight of 47 and 114 kDa,(More)
Alcaligenes sp. MTCC 10675 has been isolated from soil sample using enrichment method and has nitrilase catalytic system which is highly specific for the hydrolysis of arylaliphatic nitriles. Optimization of culture conditions using response surface methodology and inducer-mediated approach enhanced arylacetonitrilase production significantly (2.4-fold).(More)
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