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The epigenetic mechanisms that enable lifelong neurogenesis from neural stem cells (NSCs) in the adult mammalian brain are poorly understood. Here, we show that JMJD3, a histone H3 lysine 27 (H3K27) demethylase, acts as a critical activator of neurogenesis from adult subventricular zone (SVZ) NSCs. JMJD3 is upregulated in neuroblasts, and Jmjd3 deletion(More)
While thousands of long noncoding RNAs (lncRNAs) have been identified, few lncRNAs that control neural stem cell (NSC) behavior are known. Here, we identify Pinky (Pnky) as a neural-specific lncRNA that regulates neurogenesis from NSCs in the embryonic and postnatal brain. In postnatal NSCs, Pnky knockdown potentiates neuronal lineage commitment and expands(More)
Long non-coding RNAs (lncRNAs) comprise a diverse class of transcripts that can regulate molecular and cellular processes in brain development and disease. LncRNAs exhibit cell type- and tissue-specific expression, but little is known about the expression and function of lncRNAs in the developing human brain. Furthermore, it has been unclear whether lncRNAs(More)
Astaxanthin (AST) is a powerful antioxidant that occurs naturally in a wide variety of living organisms. Much experimental evidence has proved that AST has the function of eliminating oxygen free radicals and can protect organisms from oxidative damage. The present study was carried out to further investigate the neuroprotective effect of AST on oxidative(More)
We consider the problem of super-resolution reconstruction (SRR) in MRI. Subpixel-shifted MR images were taken in several fields of view (FOVs) to reconstruct a high-resolution image. A novel algorithm is presented. The algorithm can be applied locally and guarantees perfect reconstruction in the absence of noise. Results that demonstrate resolution(More)
To follow the spatial and temporal evolution of hemorrhage, in vivo MRI studies of experimental spinal cord injury (SCI) were performed on 17 rats in the very acute phase (hyperacute), starting as early as 9 min and continued up to 400 min posttrauma. Axial MR images were processed slice by slice over a 21 mm length around the epicenter of the injury. The(More)
The time dependence of N-acetyl-aspartate (NAA) concentrations relative to lactate and pyruvate in the injured rat spinal cord was investigated. Segments of spinal cord from regions rostral, caudal, and at the epicenter of the injury were analyzed. NAA concentrations were determined by gas chromatography-mass spectrometry and lactate and pyruvate(More)
Sequential in vivo MRI studies of experimental spinal cord injuries (SCI) were performed using a three-dimensional implementation of the FATE (Fast low-Angle spin echo sequence with short TE) sequence. MRI-observed pathology was quantified using a multispectral segmentation algorithm. Neurological analysis was performed on the same animals concurrently, in(More)
Longitudinal in vivo magnetic resonance imaging (MRI) of moderately injured rat spinal cord was performed to investigate the transient loss in gray matter (GM) and white matter (WM) contrast. In addition, neurobehavioral scores (based on open field walking, inclined plane test, and grid walking) and tissue cholesterol concentrations at pre-determined time(More)
Synthetic in vivo molecular 'computers' could rewire biological processes by establishing programmable, non-native pathways between molecular signals and biological responses. Multiple molecular computer prototypes have been shown to work in simple buffered solutions. Many of those prototypes were made of DNA strands and performed computations using cycles(More)