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Epidemiological data have demonstrated rapid growth of HIV-1 infections among injecting drug users (IDUs) in the Ukraine and Russia, during 1996. Here we describe the results of genetic analysis of isolates derived from 12 HIV-1-infected IDUs in different sites of Russia and the Ukraine. The blood samples were taken within a 1- to 2-month period after the(More)
At room temperature, HIV-1 IIIB is shown to remain infectious in a dose of 25 mg/ml of heroin solution for more than 8 days. The large batch of HIV-1-contaminated heroin solution may therefore remain infectious for a long period sufficient for transportation, packing, and sale in any area of Russia. At the same time 41-day incubation under the same(More)
The prevalence of genetic variants (subtypes) of hepatitis C virus (HCV) among HIV-infected and noninfected intravenous drug users (IDU) in Russia is studied. HIV and HCV infections spread in the population of IDUs in Russia irrespective of each other. HCV subtypes 1b, 2a, and 3a (35.2, 18.3, and 29.6%, respectively) are the most prevalent in Russia. The(More)
A fragment of the genome of human immunodeficiency virus type 1 (HIV-1) coding for p23 protein, the product of vif gene, was cloned in a plasmid vector pUR291. The resulting recombinant plasmid pLacVif1 was conducive in E. coli cells to the synthesis of a hybrid polypeptide with molecular weight of 136 kDa containing antigenic determinants of p23 protein of(More)
The frequency of anti-CD4 antibodies was determined in the sera or plasma derived from the patients infected with HIV-1 belonging to different genetic subgroups. The anti-CD4-antibodies in a dilution of > or = 1:1000 were found in 14% of the patients infected with the gagA/envA virus characteristic for injectable drug users in East Europe. The frequency of(More)
A humoral immune response to individual hepatitis C virus (HCV) antigens was studied in 49 patients at the subclinical stage of HIV-1 infection. These patients, as compared with a group comprising 50 patients with chronic hepatitis C, showed statistically significant higher levels of HCV-specific immunoglobulins G to nucleocapsid protein and the antigens(More)
The prevalence of human T-lymphotropic type I virus (HTLV-I) among patients with sexually-transmitted diseases is studied in Russia. Primary screening of antibodies to HTLV-I in the sera was carried out by enzyme immunoassay with recombinant gag and env HTLV-I-specific antigens synthesized in Escherichia coli. For secondary screening, Serodia HTLV-I and(More)
Specific features of human immunodeficiency virus type 1 (HIV-1) transmission among injecting drug users were studied on HIV infection outbreak in Lysva, the Perm region. During the period from November 1998 to March 2000, 32 injecting drug users infected with the subtype A HIV-1 variant originating from the same source, were found in this town. To(More)
The stability of human immunodeficiency virus, type 1 (HIV-1), strain IIIB, was studied in liquid preparations of homemade drugs. The "Vint" preparation (containing Methamphetamine and obtained from Ephedrine) as well as "Khanka" (a liquid surrogate opiate made from poppy straw) were analyzed within the case study. HIV-1/IIIB was shown to maintain its(More)
AIM Study safety, reactogenicity, immunogenicity and prophylactic effectiveness of polysaccharide pneumococcus vaccines during immunization of adult HIV-infected patients. MATERIALS AND METHODS 200 HIV-infected patients at stages 3 to 4A of the disease aged 20 to 50 years with the quantity of CD4+ T-lymphocytes in blood of no less than 500 microl(-1) took(More)