S. I. Chizhova

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A methodology is developed for revealing and identifying genetically modified sources (GMSs) in plant raw material and food products obtained from it, which enables routine analyses. For optimization suitable for testing DNA, three methods of isolating it are compared: CTAB method, DNA-BioPro method with Wizard columns, and Silica M method with specific(More)
The possibility to assess the growth-retarding activity of triazole compounds, such as paclobutrazole, uniconazole, and azovite, by an express bioassay based on the evaluation of the α-amylase activity, which hydrolyzes starch into soluble sugars in the aleurone layer of barley (Hordeum vulgare L.), was investigated. The retarding effects of triazoles, when(More)
Effects of triazolium compounds (paclobutrazol, uniconazole, and azovit), applied under laboratory and greenhouse conditions, on growth of barley plants (Hordeum vulgare L., cvs. Moskovskii 2 and Zazerskii 85) and ABA content in their tissues were studied for the first time. At various application techniques, the retardants suppressed growth of axial(More)
A method of multiplex polymerase chain reaction (PCR) followed by the hybridization on a hydrogel oligonucleotide biochip was developed for simultaneous identification of ten different transgenic elements of plant DNA in feed and food products. The biochip contained 22 immobilized probes intended for (i) detection of plant DNA; (ii) plant species(More)
A method of multiplex polymerase chain reaction (PCR) followed by hybridization on a hydrogel oligonucleotide biochip was developed for simultaneous identification of ten different transgenic elements of plant DNA in food and feed products. The biochip contained 22 immobilized oligonucleotide probes that were intended for (1) detection of plant DNA, (2)(More)
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