S. I. Alikhanian

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HE genetic structure of Streptomyces coelicolor A3 (2), the only strain among the actinomycetes for which a genetic map has been constructed, was studied in detail by HOPWOOD (1967) and SERMONTI (1 969). For genetic analysis, HOPWOOD and SERMONTI utilized the capacity of mutant strains of A3 (2) to undergo genetic recombination. To enlarge the possibilities(More)
Protoplasts of the entomopathogenic bacterium Bacillus thuringiensis subsp. galleria were transformed by plasmid pBC16. The frequency of transformation was much lower than that of Bacillus subtilis. All isolated B. thuringiensis transformants were characterized by increased sensitivity to lysozyme as compared with the original strain.
A new strain, Streptomyces rimosus LS-T hybrid, characterized by a lower level of foaming and higher antibiotic activity as compared to the other active strains of S. rimosus, is obtained as a result of selection among prototrophic recombinant forms of strains LS-T293 and BS-21. The studies on strain LS-T hybrid show the possibilities of combining(More)
We used the hybrid plasmid pAS8 in order to conduct the genetic analysis of RP4 plasmid. The presence of two replicons in the hybrid plasmid permitted to expand the spectrum of deletion mutants of RP4 isolated, which are capable to autonomous replication. The shortening of the hybrid plasmid was achieved by P22 transduction, by induction of deletion mutants(More)
In this paper we describe the preparation of hybrid plasmid consisting of ColE1 DNA and DNA of R6K plasmid. ColE1 plasmid represents a circular DNA with the molecular weight of 4,2-10(6) daltons. It determines colicine synthesis E1, has relaxed control of replication and is present in the cell in several dozens copies. Transmissive plasmid B6K represents a(More)