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The rigorous characterization of distinct induced pluripotent stem cells (iPSC) derived from multiple reprogramming technologies, somatic sources, and donors is required to understand potential sources of variability and downstream potential. To achieve this goal, the Progenitor Cell Biology Consortium performed comprehensive experimental and genomic(More)
21) at 37 8C for 30 min and subjected to phenol–chloroform extraction, and RNA was recovered by ethanol precipitation. RNAs were separated by 10% PAGE and analysed by northern blotting with [a-32 P]GTP-labelled antisense U1, U2, U4, U5 and U6 snRNA probes 25. Gel-shift assays were done as described 17 except that b-globin RNA was used in the splicing(More)
The use of induced pluripotent stem cells (iPSC) derived from independent patients and sources holds considerable promise to improve the understanding of development and disease. However, optimized use of iPSC depends on our ability to develop methods to efficiently qualify cell lines and protocols, monitor genetic stability, and evaluate self-renewal and(More)
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