S E Levenson

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Smooth muscle cells with 4C (double diploid) DNA content have been found in major arteries. The proportion of 4C cells increases with normal aging and with hypertension. These cells may represent a state of arrest at the G2 phase of the cell cycle or may be examples of true tetraploidy. Flow cytometric cell sorting was used to isolate 4C smooth muscle cells(More)
The smooth muscle cell population in major arteries of humans and experimental animals is heterogeneous with regard to cellular DNA content. A proportion of cells has polyploid DNA content and this proportion increases with normal aging and with hypertension. We have isolated pure populations of rat aortic smooth muscle cells containing 2C, 4C, and 8C DNA(More)
Abnormalities of growth regulation in arterial smooth muscle cells (SMC) are important in the pathogenesis of vascular disease. Recent studies have demonstrated an accumulation of polyploid SMC in hypertensive, and to a lesser extent in normotensive, arteries. The aim of this study was to evaluate the intrinsic genetic predisposition of aortic SMC from(More)
Late damage to normal tissues is an important consideration in determining the dose of radiation which can be delivered to a given target volume in clinical radiation therapy. The response of large blood vessels to radiation injury is undoubtedly complex and is influenced by (1) the cellular composition of the vessel wall, (2) the slow turnover of vascular(More)
Vascular injury has been suggested as a possible mechanism for late radiation damage to various organs. However, little is known about the radiobiology of cells of the blood vessel wall. We have studied the radiation survival properties of exponentially growing cultures of vascular smooth muscle cells. Medical smooth muscle cells were obtained from the(More)
Smooth muscle cell (SMC) growth may play an important role in the pathogenesis of vascular diseases such as atherosclerosis and hypertension. Recent studies have demonstrated that, under different growth stimuli in vivo, SMC may respond by proliferation of diploid cells, polyploidization to the tetraploid (or even octaploid) state, or both. In this study,(More)
Molecular mechanisms involved in wound healing acceleration by Staphylococcus aureus peptidoglycan were investigated with the use of polyvinyl alcohol sponges implanted under the dorsal skin of rats. Total collagen and RNA content and messenger RNA levels of alpha1(I) and alpha1(III) procollagen, transforming growth factor-beta1, and matrix(More)
Confluent cultures of rat aortic smooth muscle cells demonstrated dose-dependent potentially lethal damage repair (PLDR), with an average repair factor of 4 for a 10 Gy dose. PLDR occurred with a half-time of 2 hours and was nearly maximal by 8 h. Among other factors, PLDR may contribute to the radioresistance of large blood vessels.
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