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Stromal-vascular cells from the epididymal fat pad of 4-week-old rats, when cultured in a medium containing insulin or insulin-like growth factor, IGF-I, triiodothyronine and transferrin, were able to undergo adipose conversion. Over ninety percent of the cells accumulated lipid droplets and this proportion was reduced in serum-supplemented medium. The(More)
Stromal-vascular cells from the inguinal fat tissue of human (age range 1.5 month-27 years), were able to undergo adipose conversion when cultured in a medium containing insulin, transferrin and triiodothyronine. Between 10 and 20 per cent of the cells changed their morphology and accumulated lipid droplets within 10 to 15 days. In most cultures,(More)
Polyclonal antibodies have been raised in rabbits against homogeneous lipoprotein lipase (LPL) purified from the media of adipose 3T3-F442A cells. The antibody is able to inhibit the apolipoprotein C-II-dependent activity of LPL, to immunoprecipitate LPL under nondenaturating conditions from media and cellular extracts. A dot-blot immunoassay of secreted(More)
Friend erythroleukemia cells, a widely used in vitro model of murine erythropoiesis, express prior to induction, a state of erythroid differentiation similar to that of the early erythroblast in vivo. To investigate whether this uniform and stable epigenetic state was the result of a selection in long-term culture for the corresponding cell type, 29 new(More)
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