S. D. Razumovskii

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Oxidation of arachidonic acid by ROS in vitro can be evaluated by the formation of reaction products (conjugated dienes); this is preceded by a lag period caused by the action of antioxidants (alpha-tocopherol, beta-carotene, and ascorbic acid). In case of ozone titration the oxidizer is consumed even during the lag period, when conjugated dienes are not(More)
Using an original automated analyzer of double bonds we determined the rate constants for oxidation of saturated and unsaturated mono- and dienoic fatty acids (in vivo substrates for beta-oxidation in the mitochondria) by the ozone titration method. The rate constant for O(3) oxidation is maximum for oleic monoenoic acid, lower for dienoic linoleic, and(More)
Presented in this paper is the sterilization method of the assisted circulation unit. The method has been approved by stand testing and then applied for sterilizing the unit Ae, yeK-5 m when experimental implanting artificial heart. According to the method, ozone in concentration of 5 x 10(-3) moles/1 and with flow rate of 0,5 1/min is passed through an(More)
139 Ozone as a representative of reactive oxygen spee cies (ROS) is one of the most toxic components of the atmosphere. A number of studies have shown that ozone is able to generate other ROS, including HO • , , H 2 O 2 , etc. [1, 2]. In some cases, these secondary ROS can be even more deleterious than the ozone molecules themselves. It is now generally(More)
213 Fibrinnstabilizing factor (FXIII) belongs to the family of transglutaminases (endooγglutamine:ε lysine transferase, EC. 2.3.2.13) and is one of the key proteins of the blood coagulation system. Similarly to other blood clotting factors, FXIII circulates in plasma as an inactive precursor. This is a heterotett ramer (FXIIIIA 2 B 2) with a molecular(More)
Ozone-induced free-radical oxidation of fragments D and E from fibrinogen has been studied. The methods of elastic and dynamic light scattering in combination with electrophoresis of unreduced samples have shown the acceleration of enzymatic covalent crosslinking of molecules of oxidation-modified fragment D under the action of factor XIIIa. UV and IR(More)
The mechanism of enzymatic covalent crosslinking of fibrinogen molecules under the effect of plasma transglutaminase (factor XIIIa) has been studied. Using the methods of elastic and dynamic light scattering combined with viscosimetry and electrophoresis of the reduced samples, it has been shown that fibrinogen oxidation strongly accelerates self-assembly(More)