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The mechanism of self-assembly of fibrin monomers and fibrinogen aggregation during ozone oxidation has been studied by the methods of elastic and dynamic light-scattering and viscosimetry. Fibrin obtained from oxidized fibrinogen exhibits higher average fiber mass/length ratio compared with native fibrin. Fibrinogen ozonation sharply reduced the latent(More)
Oxidation of arachidonic acid by ROS in vitro can be evaluated by the formation of reaction products (conjugated dienes); this is preceded by a lag period caused by the action of antioxidants (α-tocopherol, β-carotene, and ascorbic acid). In case of ozone titration the oxidizer is consumed even during the lag period, when conjugated dienes are not yet(More)
Using an original automated analyzer of double bonds we determined the rate constants for oxidation of saturated and unsaturated mono- and dienoic fatty acids (in vivo substrates for β-oxidation in the mitochondria) by the ozone titration method. The rate constant for O3 oxidation is maximum for oleic monoenoic acid, lower for dienoic linoleic, and very low(More)
Presented in this paper is the sterilization method of the assisted circulation unit. The method has been approved by stand testing and then applied for sterilizing the unit Ae, yeK-5 m when experimental implanting artificial heart. According to the method, ozone in concentration of 5 x 10(-3) moles/1 and with flow rate of 0,5 1/min is passed through an(More)
20 Fibrinogen is a multidomain blood plasma protein, the main function of which is to produce insoluble fibrin. Fibrinogen molecule is a dimer comprised of three pairs of polypeptide chains ( A α , B β , and γ ) 2 . The central part of the molecule, encompassing the NH 2 terminal regions of all polypeptide chains, forms the central domain E , which contains(More)
The ability of a water-soluble inclusion complex (KN-14-CD) of an aminomethylated dihydroquercetin derivative (KN-14) in β-cyclodextrin (CD) to inhibit ozone-induced oxidation of fibrinogen was evaluated. KN-14-CD prevented ozone-induced oxidation of fibrinogen and preserved its ability to form clots after the addition of thrombin. The ability of KN-14-CD(More)
The mechanism of enzymatic covalent crosslinking of fibrinogen molecules under the effect of plasma transglutaminase (factor XIIIa) has been studied. Using the methods of elastic and dynamic light scattering combined with viscosimetry and electrophoresis of the reduced samples, it has been shown that fibrinogen oxidation strongly accelerates self-assembly(More)
139 Ozone as a representative of reactive oxygen spee cies (ROS) is one of the most toxic components of the atmosphere. A number of studies have shown that ozone is able to generate other ROS, including HO • , , H 2 O 2 , etc. [1, 2]. In some cases, these secondary ROS can be even more deleterious than the ozone molecules themselves. It is now generally(More)
315 Fibrinogen is a soluble plasma protein that plays the key role in fibrinogenesis. This is a dimer consisting of three pairs of polypeptide chains ( A α , B β , γ ) 2 , which form five major domains: the central domain E, two peripheral domains D, and two domains α C. The last two domains are the C-terminal regions of A α chains. Domains E and D are(More)