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The time course and pattern of incorporation of rhodamine-labeled actin microinjected into cultured fibroblastic cells were examined by fluorescence microscopy. Following microinjection, the fluorescent probe was incorporated rapidly into ruffling membranes, and within 5 min faintly fluorescent stress fibers were observed. Levels of fluorescence in ruffling(More)
Microinjection of skeletal actin labeled with rhodamine into cultured cardiac myocytes was followed by rapid incorporation of fluorescence into myofibrils of the cells. Myocytes examined as shortly as 5 min postinjection displayed fluorescent bands corresponding to the sarcomeres. By 10 min, distinct alternating wide and narrow bands of fluorescence were(More)
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